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Multiparameter flow cytometric analysis of TREM-1(CD354) expression on human peripheral blood leucocytes. Whole blood was stained with either PE Mouse IgG1 κ Isotype Control (Cat. No. 554680; Left Panel) or PE Mouse Anti-Human TREM-1 (CD354) antibody (Cat. No. 565555; Right Panel). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter flow cytometric contour plots showing the correlated expression of TREM-1 (CD354) [or Ig Isotype control staining] versus side-light scatter (SSC) signals were derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.
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BD Pharmingen™ PE Mouse Anti-Human TREM-1 (CD354)
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Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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The 6B1monoclonal antibody specifically binds to TREM-1 (Triggering Receptor Expressed on Myeloid Cells 1 or Triggering Receptor Expressed on Monocytes 1) which is also known as CD354. CD354 is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily. CD354 is highly expressed by neutrophils and monocytes. It is also reportedly expressed at lower levels by a variety of cells including subsets of T and B cells, NK cells and dendritic cells. Its expression is upregulated on leucocytes in response to microbial lipopolysaccharide, eg, during microbial infections or sepsis. CD354 activation triggers the release of proinflammatory cytokines and chemokines. A soluble form of CD354 has been described that may play a protective role in some infectious disease states.

Development References (5)
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Bouchon A, Dietrich J, Colonna M. Cutting edge: inflammatory responses can be triggered by TREM-1, a novel receptor expressed on neutrophils and monocytes. J Immunol. 2000; 164(10):4991-4995. (Biology). View Reference
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Ford JW, McVicar DW. TREM and TREM-like receptors in inflammation and disease. Curr Opin Immunol. 2009; 21(1):38-46. (Biology). View Reference
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Haselmayer P, Grosse-Hovest L, von Landenberg P, Schild H, Radsak MP. TREM-1 ligand expression on platelets enhances neutrophil activation. Blood. 2007; 110(3):1029-1035. (Immunogen: Functional assay, Inhibition, Neutralization). View Reference
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Matesanz-Isabel J, Sintes J, Llinas L, de Salort J, Lazaro A, Engel P. New B-cell CD molecules. Immunol Lett. 2011; 134(2):104-112. (Biology). View Reference
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Radsak MP, Taube C, Haselmayer P, et al. Soluble triggering receptor expressed on myeloid cells 1 is released in patients with stable chronic obstructive pulmonary disease. Clin Dev Immunol. 2007; 52040:1-7. (Immunogen: ELISA, Flow cytometry). View Reference
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