FlowJo™ v10 Software

The leading analysis platform for single-cell flow and mass cytometry analysis

Overview

FlowJo™ Software is your partner and committed to take your research to new heights.

FlowJo™ Software enables single-cell flow cytometry analysis that helps unlock your data insights with accessible features for immunophenotyping, cell cycle, proliferation, kinetics studies, quantitative population comparison, plate screening assays and more.

FEATURES

With an intuitive interface, specialized analysis platforms and open-ended plugin architecture, FlowJo™️ Software provides a rich analysis environment that is easy to use, versatile and extensible.

Analyzes any .fcs file from any cytometer
Features intuitive drag-and-drop analysis
Includes tools with which to analyze high-parameter data
Allows experiment-based analysis
Provides publication-quality graphics
Has plugin architecture that allows for new tools to be released often

The FlowJo™ Software workspace is a powerful statistical environment that is used for immunophenotyping, cell cycle, proliferation, kinetics studies, quantitative population comparison or plate screening assays.

Easily continue your analysis workflow with built-in BD FACSDiva™ Software integration. BD FACSDiva™ Software experiments can now be imported directly into FlowJo™ Software, which can also export FACSDiva™ Software templates.

Open-ended plugin architecture helps your research stay ahead of the curve. FlowJo™ Software offers easy plugin installation that brings ongoing informatics innovation to your data visualizations.

Our developers work with the brightest minds in the industry to cultivate these cutting-edge resources. Current offerings include but are not limited to:

Phenograph
ClusterExplorer
UMAP
CytoNorm
FlowSOM

Download and see the complete library of plugins available on the FlowJo Exchange!

With FlowJo Portal you can ditch the Dongle and access your analysis with your own username and password.

With FlowJo Portal you can ditch the Dongle and access your analysis with your own username and password. With a FlowJo Portal annual subscription license to FlowJo™ Software, you get:

Automatic access to new version releases
The ability to sign in to FlowJo™ Software on any computer with internet connectivity
One account to access FlowJo™ and SeqGeq™ Software licenses

New Updates

The latest release of FlowJo™ v10.9 Software

Embed—Forward propagate your clustering or dimensionality reduction results to new files with the same parameters.
Marker Enrichment Modeling (MEM)—Name your clusters based on their expression with a human-and machine-readable label.
Extract Autofluorescence without Autospill—Use an unstained cell data file to model autofluorescence for subtraction.
- Automatically create quads and rectangle gates based on range gates, bi-sector gates or the data itself.
- Also, gates with the same name can now be linked together as ‘clones’ so that their position is synchronized.

Built-in ClusterExplorer—Display line charts, bar charts, heat maps and dimensionality reduction plots of clustered data in FlowJo^™ Software without the use of a plugin.
Spectral similarity scores—Calculate the cosine index for your actual data to evaluate reagent choices you made in panel design.
Improved interaction with BD^® Research Cloud (BD RC)—BD RC is a new tool for collaboration, experiment and data management, panel design and more.

TRAINING AND RESOURCES

Basic Tutorial >	Download FlowJo^™ v10 Software >
FlowJo Exchange >	FlowJo University >
Webinars >	Documentation >