The F10/21A3 monoclonal antibody specifically binds to CD1c. The CD1 family of transmembrane glycoproteins are structurally related to the classical major histocompatibility complex (MHC) proteins. CD1c is a type I transmembrane glycoprotein that forms heterodimers with beta-2-microglobulin. CD1c presents lipids and glycolipids of self or microbial origin to T cells. CD1c is expressed by Langerhans cells, dendritic cells, monocytes, cortical thymocytes, T cells, and some B cells.
The antibody was conjugated to BD Horizon™ BB700, which is part of the BD Horizon Brilliant™ Blue family of dyes. It is a polymer-based tandem dye developed exclusively by BD Biosciences. With an excitation max of 485 nm and an emission max of 693 nm, BD Horizon BB700 can be excited by the 488 nm laser and detected in a standard PerCP-Cy™5.5 set (eg, 695/40-nm filter). This dye provides a much brighter alternative to PerCP-Cy5.5 with less cross laser excitation off the 405 nm and 355 nm lasers.