Multiparameter flow cytometric analysis of CD75/CD75s expression on human peripheral blood leucocyte populations. Peripheral blood was treated with BD Pharmingen Cell Lysis Buffer (Cat. No. 559759) to lyse erythrocytes and washed. The leucocytes were then stained with either Alexa Fluor® 647 Mouse IgM, κ Isotype Control (Cat. No. 560806; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CD75/CD75s antibody (Cat. No. 566350; Right Plot). Flow cytometric contour plots showing the correlated expression of CD75/CD75s (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
Two-color flow cytometric analysis of CD75/CD75s expression on human peripheral blood lymphocytes. Peripheral blood was treated with BD Pharmingen Cell Lysis Buffer (Cat. No. 559759) to lyse erythrocytes and washed. The leucocytes were then stained with BD Horizon™ BV421 Mouse Anti-Human CD19 antibody (Cat. No. 562440/562441) and either Alexa Fluor® 647 Mouse IgM, κ Isotype Control (Cat. No. 560806; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CD75/CD75s antibody (Cat. No. 566350; Right Plot).The flow cytometric contour plots showing the correlated expression of CD75/CD75s (or Ig Isotype control staining) versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
Multiparameter flow cytometric analysis of CD75/CD75s expression on human peripheral blood leucocyte populations. Peripheral blood was treated with BD Pharmingen Cell Lysis Buffer (Cat. No. 559759) to lyse erythrocytes and washed. The leucocytes were then stained with either Alexa Fluor® 647 Mouse IgM, κ Isotype Control (Cat. No. 560806; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CD75/CD75s antibody (Cat. No. 566350; Right Plot). Flow cytometric contour plots showing the correlated expression of CD75/CD75s (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
Two-color flow cytometric analysis of CD75/CD75s expression on human peripheral blood lymphocytes. Peripheral blood was treated with BD Pharmingen Cell Lysis Buffer (Cat. No. 559759) to lyse erythrocytes and washed. The leucocytes were then stained with BD Horizon™ BV421 Mouse Anti-Human CD19 antibody (Cat. No. 562440/562441) and either Alexa Fluor® 647 Mouse IgM, κ Isotype Control (Cat. No. 560806; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CD75/CD75s antibody (Cat. No. 566350; Right Plot).The flow cytometric contour plots showing the correlated expression of CD75/CD75s (or Ig Isotype control staining) versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
Product Details
BD Pharmingen™
CD75/CD75s; CDw75
Human (QC Testing)
Mouse IgM, κ
Human CD32 Peptides
Flow cytometry (Routinely Tested)
5 µl
VI CDw75.1; VII 70482
AB_2869751
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
Lysing Solution 10X Concentrate IVD
Size
100 mL
Cat No.
349202
Cell Lysis Buffer RUO
Size
50 mL
Cat No.
559759
Alexa Fluor® 647 Mouse IgM, κ Isotype Control RUO
Size
0.1 mg
Cat No.
560806
566350 Rev. 1
Antibody Details
ZB55
The ZB55 monoclonal antibody specifically recognizes the carbohydrate CD75 (lactosamines) and CD75s (sialylated lactosamines) antigens which are expressed in association with various glycolipids and glycoproteins. CD75 is expressed on mature B cells and germinal center B lymphocytes whereas CD75s is expressed on mature B cells. CD75 expression is lost on B cells differentiating to plasma cells whereas CD75s expression is lost on both germinal center B cells and B cells differentiating into plasma cells. CD75 and CD75s may be expressed by some T cells and can be useful markers for distinguishing different types of malignant B cells.
566350 Rev. 1
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
566350 Rev.1
Citations & References
View product citations for antibody "566350" on CiteAb
Development References (7)
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CDw75 Workshop Panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:169-171.
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Distler U, Souady J, Hülsewig M, et al. Tumor-associated CD75s- and iso-CD75s-gangliosides are potential targets for adjuvant therapy in pancreatic cancer.. Mol Cancer Ther. 2008; 7(8):2464-75. (Biology). View Reference
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Jones M, Mason DY. B-cell Antigens: Section report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:127-130.
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Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
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Schwartz-Albiez R. Carbohydrate and lectin: Section report. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:149-164.
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Stamenkovic I, Sgroi D, Aruffo A, Sy MS, Anderson T. The B lymphocyte adhesion molecule CD22 interacts with leukocyte common antigen CD45RO on T cells and alpha 2-6 sialyltransferase, CD75, on B cells. Cell. 1991; 66(6):1133-1144. (Biology). View Reference
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Zola H, Swart B, Nicholson I, Voss E. CD75, CD75s, CDw76. In: Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007:168.
566350 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.