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BD Transduction Laboratories™ Purified Mouse Anti-Rsk
Clone 78/RSK (RUO)
Western blot analysis of Rsk on A431 cells lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-Rsk antibody.
Immunofluorescent staining of Hs 766T cells.
Western blot analysis of Rsk on A431 cells lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-Rsk antibody.
Immunofluorescent staining of Hs 766T cells.
Product Details
BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
Mouse IgG2a
Human p90[rsk] aa. 1-184
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
90 kDa
250 µg/ml
AB_397622
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610225 Rev. 1
Antibody Details
78/RSK
The p90[rsk] (Rsk) and p70[s6k] kinases were first identified based on their ability to phosphorylate the 40S ribosomal protein S6 in vitro. Both of these enzymes are differentially regulated by serine/threonine phosphorylation in response to mitogenic stimulation. ERK1 and ERK2 have been shown to regulate Rsk activity. Once activated by this phosphorylation, a significant amount of Rsk can be found in the nucleus, suggesting that it has a role in nuclear signaling events. The regulation of nuclear Rsk and ERK activities by growth factors is coordinated with the induction of several early response genes. Rsk has also been shown to be activated by ionizing radiation, presumably through an activated MAP kinase. Studies in Xenopus oocytes and mouse NIH/3T3 cells indicate that inactive Rsk and ERK2 exist in a complex of approximately 110kDa. Upon phosphorylation of Rsk and ERK2, the heterodimer dissociates and at least a portion of these activated kinases translocate to the nucleus.
610225 Rev. 1
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610225 Rev.1
Citations & References
View product citations for antibody "610225" on CiteAb
Development References (5)
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Chung J, Pelech SL, Blenis J. Mitogen-activated Swiss mouse 3T3 RSK kinases I and II are related to pp44mpk from sea star oocytes and participate in the regulation of pp90rsk activity. Proc Natl Acad Sci U S A. 1991; 88(11):4981-4985. (Biology). View Reference
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Hsiao KM, Chou SY, Shih SJ, Ferrell JE Jr. Evidence that inactive p42 mitogen-activated protein kinase and inactive Rsk exist as a heterodimer in vivo. Proc Natl Acad Sci U S A. 1994; 9(12):5480-5484. (Biology). View Reference
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Majka M, Janowska-Wieczorek A, Ratajczak J. Stromal-derived factor 1 and thrombopoietin regulate distinct aspects of human megakaryopoiesis. Blood. 2000; 96(13):4142-4151. (Clone-specific: Western blot). View Reference
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Moor AN, Gan XT, Karmazyn M, Fliegel L. Activation of Na+/H+ exchanger-directed protein kinases in the ischemic and ischemic-reperfused rat myocardium. J Biol Chem. 2001; 276(19):16113-16122. (Clone-specific: Western blot). View Reference
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Morrione A, Navarro M, Romano G. The role of the insulin receptor substrate-1 in the differentiation of rat hippocampal neuronal cells. Oncogene. 2001; 20(35):4842-4852. (Clone-specific: Western blot). View Reference
610225 Rev. 1
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.