Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Ran is a highly conserved GTPase that is ubiquitously expressed. At steady-state, 80-90% of cellular Ran is located in the nucleus, with the remainder in the cytoplasm. The human TC4/Ran cDNA was originally isolated based on the predicted homology of its encoded protein product to Ras. Unlike other members of the Ras superfamily, Ran does not appear to contain signals for lipid modification, nor does it appear to show membrane localization. Ran has been implicated in a number of cellular processes such as the initiation of DNA replication, entry into and exit from mitosis and in nuclear RNA and protein transport through the nuclear pore complex. A number of Ran binding proteins have been identified. One of the smaller proteins identified is a 28kDa cytosolic molecule known as RanBP1. The GTP-bound state of Ran is stabilized through its binding to RanBP1.
Development References (5)
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Drivas GT, Shih A, Coutavas E, Rush MG, D'Eustachio P. Characterization of four novel ras-like genes expressed in a human teratocarcinoma cell line. Mol Cell Biol. 1990; 10(4):1793-1798. (Biology). View Reference
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Faleiro L, Lazebnik Y. Caspases disrupt the nuclear-cytoplasmic barrier. J Cell Biol. 2000; 151(5):951-959. (Clone-specific: Immunofluorescence). View Reference
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Iborra FJ, Jackson DA, Cook PR. The path of RNA through nuclear pores: apparent entry from the sides into specialized pores. J Cell Sci. 2000; 113(2):291-302. (Clone-specific: Electron microscopy). View Reference
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Nemergut ME, Lindsay ME, Brownawell AM, Macara IG. Ran-binding protein 3 links Crm1 to the Ran guanine nucleotide exchange factor. J Biol Chem. 2002; 277(20):17385-17388. (Clone-specific: Western blot). View Reference
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Schwoebel ED, Ho TH, Moore MS. The mechanism of inhibition of Ran-dependent nuclear transport by cellular ATP depletion. J Cell Biol. 2002; 157(6):963-974. (Clone-specific: Immunofluorescence, Western blot). View Reference
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