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Purified Mouse Anti-M-Cadherin
Product Details
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BD Transduction Laboratories™
Mouse (QC Testing), Rat (Tested in Development)
Mouse IgG2a
Mouse M-Cadherin aa. 253-366
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
130 kDa
250 µg/ml
AB_398414
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
611100 Rev. 1
Antibody Details
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5/M-Cadherin

Cadherins are a family of transmembrane glycoproteins involved in the Ca2+-dependent cell-cell adhesion that occurs in many tissues. Members of this family include P-Cadherin, E-Cadherin (uvomorulin), N-Cadherin, R-Cadherin, Cadherin-5, L-CAM, and EP-Cadherin. These proteins are similar in their domain structure (45-74% amino acid conservation), Ca2+ and protease sensitivity, and molecular weight. However, cadherins have distinct tissue expression patterns and immunological reactivities. M (muscle)-Cadherin, another member of the Cadherin family, was discovered in myogenic mouse cells where it is present at low levels in myoblasts. It is expressed in prenatal and adult skeletal muscle and plays a role in skeletal muscle cell differentiation, particularly the fusion of myoblasts into myotubes. It is upregulated upon induction of myotube formation. M-Cadherin also forms complexes with the catenins in skeletal muscle cells, which then interact with the cytoskeleton. Therefore, it is thought that the M-Cadherin-cytoskeleton interaction may play a role in aligning myoblasts during fusion.

611100 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611100 Rev.1
Citations & References
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Development References (5)

  1. Donalies M, Cramer M, Ringwald M, Starzinski-Powitz A. Expression of M-cadherin, a member of the cadherin multigene family, correlates with differentiation of skeletal muscle cells. Proc Natl Acad Sci U S A. 1991; 88(18):8024-8028. (Biology). View Reference
  2. Kang JS, Feinleib JL, Knox S, Ketteringham MA, Krauss RS. Promyogenic members of the Ig and cadherin families associate to positively regulate differentiation. Proc Natl Acad Sci U S A. 2003; 100(7):3989-3994. (Clone-specific: Western blot). View Reference
  3. Kaufmann U, Kirsch J, Irintchev A, Wernig A, Starzinski-Powitz A. The M-cadherin catenin complex interacts with microtubules in skeletal muscle cells: implications for the fusion of myoblasts. J Cell Sci. 1999; 112(1):55-68. (Biology). View Reference
  4. Kuch C, Winnekendonk D, Butz S, Unvericht U, Kemler R, Starzinski-Powitz A. M-cadherin-mediated cell adhesion and complex formation with the catenins in myogenic mouse cells. Exp Cell Res. 1997; 232(2):331-338. (Biology). View Reference
  5. Shimoyama Y, Shibata T, Kitajima M, Hirohashi S. Molecular cloning and characterization of a novel human classic cadherin homologous with mouse muscle cadherin. J Biol Chem. 1998; 273(16):10011-10018. (Biology). View Reference
View All (5) View Less
611100 Rev. 1

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