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Purified Mouse Anti-Human DEK
Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1
Human DEK aa. 19-169
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
50 kDa
250 µg/ml
AB_398261
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610948 Rev. 1
Antibody Details
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2/DEK

The (6;9) chromosomal translocation is associated with acute myelogenousleukemia (AML) and fuses the dek and can genes. This results in expression of the oncogenic DEK-CAN fusion protein, consisting of the N-terminal two-thirds of DEK and the C-terminal two-thirds of CAN. Although, on its own, DEK exhibits anti-oncogenic properties, the DEK-CAN chimera appears to be oncogenic. DEK is a nuclear protein with a calculated molecular weight of 42-43 kD, that can be observable at 50 kD, and reportedly exhibits no substantial homology to any known protein sequences. Although it contains 42% charged amino acids and multiple acidic sequences, specific structural features have yet to be identified. In addition to its involvement in AML, DEK is associated with several disease states, such as juvenile rheumatoid arthritis where it is an autoantigen. Efforts to define the cellular function of DEK led to its identification as the pets factor. The peri-ets (pets) site is a TG-rich element between the two Elf-1 binding sites of the HIV-2 enhancer. The pets site mediates transcriptional activation in response to T cell stimulation. Thus, DEK is a site-specific DNA binding protein that functions in transcriptional regulation and signal transduction.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610948 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610948 Rev.1
Citations & References
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Development References (3)

  1. Fu GK, Grosveld G, Markovitz DM. DEK, an autoantigen involved in a chromosomal translocation in acute myelogenous leukemia, binds to the HIV-2 enhancer. Proc Natl Acad Sci U S A. 1997; 94(5):1811-1815. (Biology). View Reference
  2. Fu GK, Markovitz DM. Purification of the pets factor. A nuclear protein that binds to the inducible TG-rich element of the human immunodeficiency virus type 2 enhancer. J Biol Chem. 1996; 271(32):19599-19605. (Biology). View Reference
  3. von Lindern M, Fornerod M, van Baal S, et al. The translocation (6;9), associated with a specific subtype of acute myeloid leukemia, results in the fusion of two genes, dek and can, and the expression of a chimeric, leukemia-specific dek-can mRNA. Mol Cell Biol. 1992; 12(4):1687-1697. (Biology). View Reference
610948 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

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