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Purified Mouse Anti-Human Btk
Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG2a
Human N-Terminal Btk aa. 2-172 Recombinant Protein
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
77 kDa
250 µg/ml
AB_398428
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
611117 Rev. 1
Antibody Details
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53/BTK

Bruton's tyrosine kinase (Btk) is a nonreceptor tyrosine kinase whose function is critical for proper B cell development and signaling.  It is a member of the Tec family of kinases which includes Tec and Itk.  This family is similar to the src family of tyrosine kinases. However, Tec family members lack the N-terminal myristylation site and the regulatory C-terminal tyrosine that are found in src proteins. In addition to an N-terminal pleckstrin homology (PH) domain, the Tec proteins contain Src homology domains 2 and 3 (SH2 and SH3) and a stretch of 60-80 amino acids between the PH and SH3 domains termed the Tec homology domain.  The activity of Btk is regulated by Src-mediated phosphorylation of the kinase domain at tyrosine 551.  This event induces Btk kinase activity and subsequent autophosphorylation at tyrosine 223 in the SH3 domain.  Phosphorylated Btk then associates with the cell membrane via the interaction of the PH domain with phosphatidylinositol 3, 4, 5-triphosphate.  The PH domain is essential for proper activation and function of Btk.  A mutation in the PH domain results in Xid, murine X-linked immunodeficiency, and human X-linked agammaglobulinemia.

611117 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611117 Rev.1
Citations & References
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Development References (5)

  1. Aoki Y, Isselbacher KJ, Pillai S. Bruton tyrosine kinase is tyrosine phosphorylated and activated in pre-B lymphocytes and receptor-ligated B cells. Proc Natl Acad Sci U S A. 1994; 91(22):10606-10609. (Biology). View Reference
  2. Shahan TA, Sorenson WG, Simpson J, Kefalides NA, Lewis DM. Tyrosine kinase activation in response to fungal spores is primarily dependent on endogenous reactive oxygen production in macrophages. J Biol Chem. 2000; 275(14):10175-10181. (Clone-specific: Immunoprecipitation, In vitro kinase assay, Western blot). View Reference
  3. Sideras P, Müller S, Shiels H. Genomic organization of mouse and human Bruton's agammaglobulinemia tyrosine kinase (Btk) loci. J Immunol. 1994; 153(12):5607-5617. (Biology). View Reference
  4. Vetrie D, Vorechovský I, Sideras P. The gene involved in X-linked agammaglobulinaemia is a member of the src family of protein-tyrosine kinases. Nature. 1993; 361(6409):226-233. (Biology). View Reference
  5. Yang W, Malek SN, Desiderio S. An SH3-binding site conserved in Bruton's tyrosine kinase and related tyrosine kinases mediates specific protein interactions in vitro and in vivo. J Biol Chem. 1995; 270(35):20832-20840. (Biology). View Reference
View All (5) View Less
611117 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

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