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Purified Mouse Anti-EEA1
Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Human, Dog, Chicken (Tested in Development)
Mouse IgG1
Human EEA1 aa. 3-281
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
180 kDa
250 µg/ml
AB_397830
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610456 Rev. 1
Antibody Details
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14/EEA1

Early endosomes are a major cytoplasmic sorting compartment from which receptors and ligands may be distributed to various sites within the cell. Early endosome antigen 1 (EEA1) is a 180 kDa hydrophilic peripheral membrane protein present in cytosol and membrane fractions. Immunofluorescence studies show that EEA1 colocalizes to early endosomes with transferrin receptor and Rab5, but not with the late endosome-localizing Rab7. EEA1 is predominantly α-helical and shares 17-20% sequence identity with the myosins. It contains a calmodulin-binding IQ motif and metal-binding cysteine "finger" motifs. It is thought that EEA1 is required for vesicular transport of proteins through early endosomes and that these finger motifs are required for this activity.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610456 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610456 Rev.1
Citations & References
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Development References (5)

  1. Allan BB, Moyer BD, Balch WE. Rab1 recruitment of p115 into a cis-SNARE complex: programming budding COPII vesicles for fusion. Science. 2000; 289(5478):444-448. (Clone-specific: Immunofluorescence, Western blot). View Reference
  2. Mu FT, Callaghan JM, Steele-Mortimer O, et al. EEA1, an early endosome-associated protein. EEA1 is a conserved alpha-helical peripheral membrane protein flanked by cysteine "fingers" and contains a calmodulin-binding IQ motif. J Biol Chem. 1995; 270(22):13503-13511. (Biology). View Reference
  3. Mullock BM, Smith CW, Ihrke G, et al. Syntaxin 7 is localized to late endosome compartments, associates with Vamp 8, and Is required for late endosome-lysosome fusion. Mol Biol Cell. 2000; 11(9):3137-3153. (Clone-specific: Immunofluorescence). View Reference
  4. Yan R, Han P, Miao H, Greengard P, Xu H. The transmembrane domain of the Alzheimer's beta-secretase (BACE1) determines its late Golgi localization and access to beta -amyloid precursor protein (APP) substrate. J Biol Chem. 2001; 276(39):36788-36796. (Clone-specific: Immunofluorescence). View Reference
  5. van Dam EM, Stoorvogel W. Dynamin-dependent transferrin receptor recycling by endosome-derived clathrin-coated vesicles. Mol Biol Cell. 2002; 13(1):169-182. (Clone-specific: Immunofluorescence). View Reference
View All (5) View Less
610456 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

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