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Purified Mouse Anti-B56α
Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse, Dog, Chicken, Frog (Tested in Development)
Mouse IgG1
Human B56α aa. 1-162
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
56 kDa
250 µg/ml
AB_397947
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610615 Rev. 1
Antibody Details
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23/B56α

Reversible phosphorylation by protein kinases and phosphatases regulates key signaling pathways in eukaryotic cells. Type 2A protein phosphatases (PP2A) are composed of two regulatory and one catalytic subunit. The active PP2A is a heterotrimer, composed of A, B, and C subunits, each encoded by different genes. B56 is a new gene family that encodes α, β, and γ proteins for the B subunit. The three B56 isoforms are approximately 70% homologous to each other and more divergent within the first 251amino acids. While the α subunit is widely expressed, the γ isoform is most abundant in heart and muscle, and the β subunit is found predominantly in brain tissue. Availability of different B subunits appears to be a key determinant in the protein substrate specificity for PP2A and could influence the subcellular distribution of the holoenzyme.

610615 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610615 Rev.1
Citations & References
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Development References (1)

  1. McCright B, Virshup DM. Identification of a new family of protein phosphatase 2A regulatory subunits. J Biol Chem. 1995; 270(44):26123-26128. (Biology). View Reference
610615 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

23-22940-00