Biotin Mouse Anti-Rat IL-10

BD Pharmingen™ Biotin Mouse Anti-Rat IL-10

Name: Biotin Mouse Anti-Rat IL-10
Brand: BD Pharmingen™
SKU: 555084

Clone A5-6

(RUO)

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Product Details

Brand: BD Pharmingen™

Reactivity: Rat (QC Testing)

Isotype: Mouse IgG1, κ

Immunogen: Recombinant Rat IL-10

Application: ELISA Detection (Routinely Tested)

Concentration: 0.5 mg/ml

RRID: AB_395690

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

ELISA Detection: The biotinylated A5-6 antibody (Cat. No. 555084) can be used as the detection antibody in a sandwich ELISA for measuring rat IL-10 protein levels in conjunction with purified A5-7 antibody (Cat. No. 555083) as the capture antibody and recombinant rat IL-10 (Cat. No. 555113) as the standard. This biotinylated detection antibody should be pretitrated in the range of 0.5 to 2 mg/ml to determine its optimal concentration for ELISA. To obtain linear standard curves, doubling dilutions of rat IL-10 ranging from ~2,000 to 5 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit our web site, www.bdbiosciences.com, and go to the protocols section or the chapter on ELISA in the Immune Function Handbook. This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assay of serum or plasma samples. For measuring Rat IL-10 in serum or plasma, our BD OptEIA™ Rat IL-10 ELISA Set (Cat. No. 555134) is specially formulated and recommended.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

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Antibody Details

A5-6

The A5-6 antibody reacts with rat Interleukin-10 (IL-10). The immunogen used to generate this hybridoma was recombinant rat IL-10 protein.

555084 Rev. 1

Format Details

Biotin

Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.

Format: Biotin

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Citations & References

Development References (1)

Feng L, Tang WW, Chang JC, Wilson CB. Molecular cloning of rat cytokine synthesis inhibitory factor (IL-10) cDNA and expression in spleen and macrophages. Biochem Biophys Res Commun. 1993; 192(2):452-458. (Biology). View Reference

555084 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.