Fluorescence spillover is the fluorescent signal measured in adjacent, residual and cross laser detectors. Spectral overlap between fluorochromes can be managed through compensation or spectral unmixing to prevent data artifacts. However, even with compensation and unmixing, spectral overlap of two fluorochromes in a panel can lead to compromised resolution through spillover spreading and unmixing error, making spillover an important consideration in panel design.  

Spillover here is evaluated and ranked based on the analysis of a given fluorochrome’s full emission profile across five lasers. Fluorochromes with less cross-laser excitation are cleaner and will have lower impact on other fluorochromes.

Disclaimer: Results and conclusions shown throughout the Fluorochrome Faceoff are based on experiments performed under the conditions described. Users should evaluate reagents with their specific protocols as results may vary with different experimental conditions.