Skip to main content Skip to navigation

 

Intended as an aid in the diagnosis of multiple myeloma and other plasma cell disorders

 

Overview

The BD OneFlow™ PCD Tube is part of the innovative BD OneFlow™ Solution, a comprehensive set of reagents, setup beads, protocols and assay templates to reproducibly set up the flow cytometer and stain, acquire and analyze patient specimens for immunophenotyping of normal and aberrant cell populations. It is built on the research and validation work of the EuroFlow™ Consortium on the characterization of hematological malignancies for improved accurate diagnosis.1

 

The BD OneFlow™ PCD Tube, when run in parallel with the BD OneFlow™ Plasma Cell Screening Tube (PCST), is intended for flow cytometric immunophenotyping of normal and aberrant plasma cells in bone marrow as an aid in the diagnosis of multiple myeloma or other plasma cell disorders.

 

Other reagents belonging to the BD OneFlow™ Solution are BD OneFlow™ Lymphoid Screening Tube (LST) BD OneFlow™ B-cell Chronic Lymphoproliferative Diseases Tube 1 (B-CLPD T1), BD OneFlow™ Plasma Cell Screening Tube (PCST)and BD OneFlow™ Acute Leukemia Orientation Tube (ALOT).

 
performance1

View video describing the BD OneFlow™ Solution on the BD FACSLyric™ Flow Cytometer.

FEATURES

The BD OneFlow™ PCD Tube reagent composition

 

AntibodyFluorochromeCloneTarget Populations
CD38FITCHB7Backbone marker. Identification of plasma cells

CD28

PEL293Identification of aberrant plasma cell populations
CD27PerCP-Cy 5.5L128Identificaiton of aberrant plasma cell populations
CD19PE-Cy 7SJ25-C1Backbone marker. Identification of plasma cells
CD117APC104D2Identificaiton of aberrant plasma cell populations
CD81APC-H7JS81Identificaiton of aberrant plasma cell populations
CD45BD Horizon V4502D1Backbone marker. Identification of plasma cells
CD138BD Horizon™ 500-CMI15Backbone marker. Identification of plasma cells

 

A full description of the utility of the antibodies chosen for the BD OneFlow™ PCD is provided in the publication by the EuroFlow Consortium.1

 

 

APPLICATIONS

Supporting Evidence

The EuroFlow “PCD panel was able to detect and fully characterize the immunophenotype of aberrant plasma cells or their normal/reactive counterparts in all the samples tested (100), as also assessed by routine diagnostic protocols performed in parallel.”1*

References

  1. van Dongen JJM, Lhermitte L, Böttcher S, et al. on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708). EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012;26(9): 1908-1975. doi: 10.1038/leu.2012.120

  2. Moloney E, Watson H, Barge D, et al. Efficiency and health economic evaluations of BD OneFlow™ Flow Cytometry Reagents for diagnosing chronic lymphoid leukemia. Cytometry B Clin Cytom. 2019;96(6):514-520. doi: 10.1002/cyto.b.21779

  3. Kalina T, Flores-Montero J, van der Velden VHJ, et al. EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols. Leukemia. 2012;26(9):1986-2010. doi: 10.1038/leu.2012.122

BD Flow Cytometers are Class 1 Laser Products.

The EuroFlow trademark is the property of the EuroFlow Consortium and cannot be reproduced or published without prior written permission from the EuroFlow coordinator (www.euroflow.org).

The BD OneFlow™ Solution is intended for professional use only.

Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.

*This publication refers to EuroFlow and is relevant to the BD OneFlow™ Solution as the BD OneFlow™ Solution demonstrated equivalent results to EuroFlow through a clinical trial.