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The BD® ELISPOT Assay is a powerful tool for detecting and analyzing individual cells that secrete a particular protein in vitro. BD® ELISPOT Reagents coupled with a high-throughput ELISPOT plate reader provide intuitive analysis of cytokines at the single-cell level.


The reagents:

  •  Provide direct analysis of frequency of cells producing cytokines and other effector molecules using an enzyme-linked immunospot (ELISPOT) assay 
  • Offer greater sensitivity than ELISAs and intracellular staining
  • Enable high-throughput analyses for direct ex vivo monitoring of T-cell function, including antigen-specific T cells
  • Offer the flexibility to choose among several formats: 
    • BD® ELISPOT Kits—ready-to-use kits with precoated BD® ELISPOT Plates for human, nonhuman primate and mouse research
    • BD® ELISPOT Reagent Sets—coat-it-yourself sets with sufficient capture antibody, detection antibody and streptavidin-horseradish peroxidase for 10 plates worth of assays (includes 10 uncoated plates)
    • BD® ELISPOT Reagent Pairs–matched antibody pairs. Sufficient capture antibody and detection antibody for 5 plates


For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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