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Intended as an aid in the diagnosis of hematological disorders 




The BD OneFlow™ LST is part of the innovative BD OneFlow™ Solution, a comprehensive set of reagents, setup beads, protocols and assay templates to reproducibly set up the flow cytometer and stain, acquire and analyze patient specimens for immunophenotyping of normal and aberrant cell populations. It is built on the research and validation work of the EuroFlow™ Consortium on the characterization of hematological malignancies for improved accurate diagnosis.1


The BD OneFlow™ LST is intended for flow cytometric immunophenotyping of normal and aberrant mature lymphocyte populations of B, T and NK cell lineages in peripheral blood, bone marrow and lymph nodes, as an aid in diagnosis of hematological disorders.


Other reagents belonging to the BD OneFlow™ Solution are BD OneFlow™ B-cell Chronic Lymphoproliferative Diseases Tube 1 (B-CLPD T1), BD OneFlow™ Plasma Cell Screening Tube (PCST), BD OneFlow™ Plasma Cell Disorders (PCD) Tube and BD OneFlow™ Acute Leukemia Orientation Tube (ALOT).


View video describing the BD OneFlow™ Solution on the BD FACSLyric™ Flow Cytometer.



The BD OneFlow™ LST reagent composition


AntibodyFluorochromeCloneTarget Populations
CD45BD Horizon V500-C2Dl (anti-HLe-1)Mature lymphocytes, B-cell precursor


PE-Cy 7SJ25-C1B-cells, and NK-cells by exclusion
CD20BD Horizon V450L27B cells and NK cells by exclusion
Anti-LambdaFITC 1-155-2Normal and clonally expanded B cells
Anti-KappaPETB28-2Normal and clonally expanded B Cells
CD38APC-H7HB7Plasma cells and B-cell precursors, Lymphoid malignancies, NK cells
CD3APCSK7T cells, B- and NK-cells by exclusion
CD4BD Horizon™ 450SK3 (Leu-3a)T cell subpopulations
CD8FITCSK1 (Leu-2a)T cell subpopulations
CD5PerCP-Cy 5.5L17F12T cell subpopulations
Anti-TCR-1PE-Cy 711F2T cell subpopulations
CD56PEMY31 (Leu-19)NK Cells


A full description of the utility of the antibodies chosen for the BD OneFlow™ LST is provided in the publication by the EuroFlow Consortium.1  




Supporting Evidence

The EuroFlow “LST detected aberrant B-, T-, or NK-cells immunophenotypes in 149/150 (99.4%) of B-CLPD and in 78/83 (94%) of T/NK-CLPD with an overall frequency of 97.4%."1


Results from the clinical trial study (BD OneFlow™ LST Instructions for Use) show that the BD OneFlow™ LST in combination with the BD OneFlow™ B-CLPD T1 gave 100% (101 of 101) overall agreement in classifying patients as having CLL (54 of 54 concordant) and in identifying patients with other B cell chronic lymphoproliferative diseases (47 out of 47 concordant) when compared with the EuroFlow system. Furthermore, interpretation of the BD OneFlow™ Solution results was highly concordant to diagnostic truth.


  1. van Dongen JJM, Lhermitte L, Böttcher S, et al. on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708). EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012;26(9): 1908-1975. doi: 10.1038/leu.2012.120

  2. Moloney E, Watson H, Barge D, et al. Efficiency and health economic evaluations of BD OneFlow™ Flow Cytometry Reagents for diagnosing chronic lymphoid leukemia. Cytometry B Clin Cytom. 2019;96(6):514-520. doi: 10.1002/cyto.b.21779

  3. Kalina T, Flores-Montero J, van der Velden VHJ, et al. EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols. Leukemia. 2012;26(9):1986-2010. doi: 10.1038/leu.2012.122

BD Flow Cytometers are Class 1 Laser Products.

The BD OneFlow™ Solution is intended for professional use only.

The EuroFlow trademark is the property of the EuroFlow Consortium and cannot be reproduced or published without prior written permission from the EuroFlow coordinator (

Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.

*This publication refers to EuroFlow and is relevant to the BD OneFlow™ Solution as the BD OneFlow™ Solution demonstrated equivalent results to EuroFlow through a clinical trial (BD OneFlow™ LST Instructions for Use).