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      Purified Rat Anti-Mouse IgG1
      Product Details
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      BD Pharmingen™
      Mouse (QC Testing)
      Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
      Pooled Mouse IgG1
      ELISA (Routinely Tested), Flow cytometry, Immunochemistry (Tested During Development)
      0.5 mg/ml
      AB_394860
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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      Recommended Assay Procedures

      Immunofluorescent staining: For flow cytometric detection of intracytoplasmic IgG1, we recommend the directly conjugated clone, FITC-conjugated mAb A85-1 (Cat. No. 553443).

      ELISA: For the sandwich mouse IgG1 ELISA, biotinylated A85-1 mAb (Cat. No. 553441) is optimal for detection with purified anti-mouse IgG1 A85-3 mAb (Cat. No. 553445) for capture.

      IHC: For Immunohistochemical staining the Biotinylated clone A85-1 is recommended, Cat. No. 550331

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      553440 Rev. 14
      Antibody Details
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      A85-1

      The A85-1 antibody reacts specifically with mouse IgG1 of Igh-Ca and Igh-Cb haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with the A85-1 monoclonal antibody. A suspension of pooled mouse IgG1 was used as the source of immunogen.

      553440 Rev. 14
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      553440 Rev.14
      Citations & References
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      View product citations for antibody "553440" on CiteAb
      No Citations Found

      No Citations are available for this Product

      553440 Rev. 14

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.