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Two-color flow cytometric analysis of CD205 (DEC-205) expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with BD Horizon™ BV421 Rat Anti-Mouse Ly-6G and Ly-6C antibody (Cat. No. 562709) and with either APC Rat IgG2a, κ Isotype Control (Cat. No. 553930; Left Plot) or APC Rat Anti-Mouse CD205 (DEC-205) antibody (Cat. No. 567500; Right Plot) at 0.25 μg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD205 (DEC-205) [or Ig Isotype control staining] versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
BD Pharmingen™ APC Rat Anti-Mouse CD205 (DEC-205)
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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- This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
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Companion Products
The NLDC-145 monoclonal antibody specifically recognizes CD205 which is also known as Dendritic and thymic Epithelial Cells, 205 kDa (DEC-205) or Lymphocyte antigen 75 (Ly-75). CD205 (DEC-205) is a ~205 kDa type 1 transmembrane glycoprotein that is encoded by Ly75 which belongs to the mannose receptor family within the C-type lectin superfamily. The extracellular region contains an N-terminal cysteine-rich domain followed by a fibronectin type II domain, 10 C-type lectin-like domains, a transmembrane region and cytoplasmic tail with an internalization sequence. CD205 (DEC-205) is highly expressed on both CD8+ and CD103+ dendritic cell subsets and Langerhans cells. It is variably expressed by some other dendritic cell types, Gr1+ bone marrow cells, B cells, T cells, thioglycolate-elicited macrophages, granulocytes, mucosal and thymic cortical epithelial cells, and brain endothelial cells. CD205 (DEC-205) serves as a pattern recognition receptor that functions as a recycling endocytic receptor. It participates in the cellular processing, presentation, and cross-presentation of foreign or self-antigens to initiate immunity or tolerance, respectively. CD205 (DEC-205) can reportedly serve as a promising target for immunotherapy including DC-targeted vaccination using anti-CD205 (DEC-205) monoclonal antibody conjugated with antigen for T cell activation.
Development References (12)
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East L, Isacke CM. The mannose receptor family.. Biochim Biophys Acta. 2002; 1572(2-3):364-86. (Clone-specific). View Reference
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Hossain MK, Wall KA. Use of Dendritic Cell Receptors as Targets for Enhancing Anti-Cancer Immune Responses.. Cancers (Basel). 2019; 11(3):E418. (Biology). View Reference
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Inaba K, Swiggard WJ, Inaba M, et al. Tissue distribution of the DEC-205 protein that is detected by the monoclonal antibody NLDC-145. I. Expression on dendritic cells and other subsets of mouse leukocytes.. Cell Immunol. 1995; 163(1):148-56. (Clone-specific: Flow cytometry). View Reference
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Jiang W, Swiggard WJ, Heufler C. The receptor DEC-205 expressed by dendritic cells and thymic epithelial cells is involved in antigen processing. Nature. 1995; 375(6527):151-155. (Clone-specific). View Reference
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Kraal G, Breel M, Janse M, Bruin G. Langerhans' cells, veiled cells, and interdigitating cells in the mouse recognized by a monoclonal antibody. J Exp Med. 1986; 163(4):981-997. (Immunogen). View Reference
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Merad M, Sathe P, Helft J, Miller J, Mortha A. The dendritic cell lineage: ontogeny and function of dendritic cells and their subsets in the steady state and the inflamed setting.. Annu Rev Immunol. 2013; 31:563-604. (Biology). View Reference
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Steinman RM. Decisions about dendritic cells: past, present, and future.. Annu Rev Immunol. 2012; 30:1-22. (Clone-specific). View Reference
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Steinman RM. Dendritic cells: understanding immunogenicity.. Eur J Immunol. 2007; 37 Suppl 1:S53-60. (Clone-specific). View Reference
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Swiggard WJ, Mirza A, Nussenzweig MC, Steinman RM. DEC-205, a 205-kDa protein abundant on mouse dendritic cells and thymic epithelium that is detected by the monoclonal antibody NLDC-145: purification, characterization, and N-terminal amino acid sequence.. Cell Immunol. 1995; 165(2):302-11. (Clone-specific: Immunoaffinity chromatography, Immunoprecipitation, Western blot). View Reference
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Vremec D, Zorbas M, Scollay R, et al. The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells. J Exp Med. 1992; 176(1):47-58. (Clone-specific: Flow cytometry, Fluorescence microscopy, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation). View Reference
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Witmer-Pack MD, Swiggard WJ, Mirza A, Inaba K, Steinman RM. Tissue distribution of the DEC-205 protein that is detected by the monoclonal antibody NLDC-145. II. Expression in situ in lymphoid and nonlymphoid tissues.. Cell Immunol. 1995; 163(1):157-62. (Clone-specific: Immunohistochemistry, Western blot). View Reference
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Wu L, Vremec D, Ardavin C, et al. Mouse thymus dendritic cells: kinetics of development and changes in surface markers during maturation. Eur J Immunol. 1995; 25(2):418-425. (Clone-specific: Flow cytometry). View Reference
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