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Purified Mouse Anti-MASH1
Purified Mouse Anti-MASH1

Western blot analysis of MASH1. Lysate from rat embryonic brain was probed with anti-MASH1 (clone 24B72D11.1, Cat. No. 556604) at concentrations of 2.0 (lane 1), 1.0 (lane 2), and 0.5 µg/ml (lane 3). MASH1 is identified as a band of ~34 kDa.

Western blot analysis of MASH1. Lysate from rat embryonic brain was probed with anti-MASH1 (clone 24B72D11.1, Cat. No. 556604) at concentrations of 2.0 (lane 1), 1.0 (lane 2), and 0.5 µg/ml (lane 3). MASH1 is identified as a band of ~34 kDa.

Product Details
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BD Pharmingen™
Rat (QC Testing), Mouse (Tested in Development)
Mouse IgG1
Rat MASH1 full length recombinant protein
Western blot (Routinely Tested), Immunochemistry (Reported)
34 kDa
0.5 mg/ml
AB_396479
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

The 24B72D11.1 antibody is recommended for western blot analysis (0.5-2.0 µg/ml). Rat embryonic brain can be used as a positive control. Neuro2A mouse neuroblastoma cells (ATCC CCL-131) may also be used as a positive control for this application.

Western blot: For more detailed information please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

The 24B72D11.1 antibody is reported in the literature to work well for immunohistochemistry.  Immunohistochemistry: For more detailed information please refer to the cited journal references and  http://www.bdbiosciences.com/support/resources/cell_biology/index.jsp

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
556604 Rev. 10
Antibody Details
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24B72D11.1

MASH1 and MASH2 (Mammalian achaete-schute Homolog 1 and 2) are basic helixloop-helix transcription factors which are mammalian homologs of the achaete-schute gene family that is required for neuronal development in Drosophila. The bHLH motif is present in many types of transcription factors including E2-2, E47, MyoD and others. MASH1 has been shown to heterodimerize with other bHLH containing transcription factors. MASH1-E12 heteromers bind to and promote transcriptional activation of muscle creatine kinase, a known target for MyoD activation. However, MASH1 appears to play its primary role during early development of the autonomic nervous system. In committed neuronal precursor cells, early expression of MASH1 activates a subset of neuron-specific genes to promote differentiation. Thus null mutations in the MASH1 gene suggest that MASH1 is a valuable marker for investigation of neural development in mammals. MASH1 is observed as an ~34 kDa protein by SDS-PAGE.

556604 Rev. 10
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
556604 Rev.10
Citations & References
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Development References (6)

  1. Hu Y, Ippolito JE, Garabedian EM, Humphrey PA, Gordon JI. Molecular characterization of a metastatic neuroendocrine cell cancer arising in the prostates of transgenic mice. J Biol Chem. 2002; 227(46):44462-44474. (Biology). View Reference
  2. Ivaniutsin U, Chen Y, Mason JO, Price DJ, Pratt T. Adenomatous polyposis coli is required for early events in the normal growth and differentiation of the developing cerebral cortex. Neural Dev. 4(3)(Biology). View Reference
  3. Johnson JE, Birren SJ, Saito T, Anderson DJ. DNA binding and transcriptional regulatory activity of mammalian achaete-scute homologous (MASH) proteins revealed by interaction with a muscle-specific enhancer.. Proc Natl Acad Sci U S A. 1992; 89(8):3596-3600. (Biology). View Reference
  4. Lo LC, Johnson JE, Wuenschell CW, Saito T, Anderson DJ. Mammalian achaete-scute homolog 1 is transiently expressed by spatially restricted subsets of early neuroepithelial and neural crest cells. Genes Dev. 1991; 5(9):1524-1537. (Biology). View Reference
  5. Ralston J, Chiriboga L, Nonaka D. MASH1: a useful marker in differentiating pulmonary small cell carcinoma from Merkel cell carcinoma. 2008; 21(11):1357-1362. (Biology). View Reference
  6. Sommer L, Shah N, Rao M, Anderson DJ. The cellular function of MASH1 in autonomic neurogenesis. Neuron. 1995; 15(6):1245-1258. (Biology). View Reference
View All (6) View Less
556604 Rev. 10

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.