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      Purified Rat Anti-Mouse CD195
      Purified Rat Anti-Mouse CD195
      Expression of mouse CD195 by stimulated mouse splenocytes. BALB/c splenocytes were stimulated for 24 hours with ConA (10 µg/ml, final concentration; Sigma). The splenocytes were cultured for additional 5 days in the presence of mouse IL-2 (100 U/ml, final concentration; Cat. No. 550069). The splenocytes were stained with purified rat anti-mouse CD195 antibody (C34- 3448), followed by biotinylated mouse anti-rat IgG2c (A92-1, Cat. No. 553909) and Streptavidin- hycoerythrin (Cat. No. 554061) following BD Pharmingen's staining protocol (see Figure, Left panel). The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed using the purified rat IgG2c isotype control (purified A23-1; Cat. No. 553982; Right panel). The quadrant markers for the bivariate dot plots were set based on the autofluorescence control and isotype control.
      Purified Rat Anti-Mouse CD195
      Expression of mouse CD195 by stimulated mouse splenocytes. BALB/c splenocytes were stimulated for 24 hours with ConA (10 µg/ml, final concentration; Sigma). The splenocytes were cultured for additional 5 days in the presence of mouse IL-2 (100 U/ml, final concentration; Cat. No. 550069). The splenocytes were stained with purified rat anti-mouse CD195 antibody (C34- 3448), followed by biotinylated mouse anti-rat IgG2c (A92-1, Cat. No. 553909) and Streptavidin- hycoerythrin (Cat. No. 554061) following BD Pharmingen's staining protocol (see Figure, Left panel). The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed using the purified rat IgG2c isotype control (purified A23-1; Cat. No. 553982; Right panel). The quadrant markers for the bivariate dot plots were set based on the autofluorescence control and isotype control.
      Product Details
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      BD Pharmingen™
      Ccr5; chemokine (C-C) receptor 5; C-C CKR-5; CC-CKR-5; CCR-5; AM4-7
      Mouse (QC Testing)
      Rat IgG2c, κ
      Mouse CCR5 aa. 9-30
      Flow cytometry (Routinely Tested)
      0.5 mg/ml
      AB_397375
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
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      Recommended Assay Procedures

      Immunofluorescent Staining and Flow Cytometry Analysis: The purified C34-3448 antibody can be used for multicolor immunofluorescent staining and flow cytometric analysis to identify and enumerate CD195-expressing cells within mixed cell populations. For optimal immunofluorescent staining with flow cytometric analysis, the antibody should be titrated (≤0.25 µg mAb/million cells, see Figure above). For specific methodology, please visit the protocols section or chapter on intracellular staining in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      559921 Rev. 2
      Antibody Details
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      C34-3448

      The C34-3448 monoclonal antibody specifically binds to CD195 which is also known as, C-C chemokine receptor type 5 (CCR5). CD195 is a seven transmembrane-spanning G-protein-coupled receptor that belongs to the β-chemokine receptor family. CD195 regulates lymphocyte chemotaxis activation and transendothelial migration during inflammation. It signals in response to at least three chemokines: CCL3/MIP-1α, CCL4/MIP-1β, and CCL5/RANTES. CD195 is expressed on macrophages and some T-lymphocytes.

      559921 Rev. 2
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      559921 Rev.2
      Citations & References
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      View product citations for antibody "559921" on CiteAb

      Development References (3)

      1. Boring L, Gosling J, Monteclaro FS, Lusis AJ, Tsou CL, Charo IF. Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors: evidence for two closely linked C-C chemokine receptors on chromosome 9. J Biol Chem. 1996; 271(13):7551-7558. (Biology). View Reference
      2. Meyer A, Coyle AJ, Proudfoot AE, Wells TN, Power CA. Cloning and characterization of a novel murine macrophage inflammatory protein-1 alpha receptor. J Biol Chem. 1996; 271(24):14445-14451. (Biology). View Reference
      3. Napolitano M, Seamon KB, Leonard WJ. Identification of cell surface receptors for the Act-2 cytokine. J Exp Med. 1990; 172(1):285-289. (Biology). View Reference
      559921 Rev. 2

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.