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BD Pharmingen™ FITC Rat Anti-Mouse Vα2 TCR
Clone B20.1 (RUO)
Flow cytometric analysis of Vα2 TCR expression on mice peripheral lymphocytes. BALB/c lymph node cells were incubated simultaneously with PE Rat Anti-Mouse CD8b.2 (Cat. No. 553041), PE Rat Anti-Mouse CD4 (Cat. No. 553730/561829/557308) and FITC Rat Anti-Mouse Vα2 TCR (Cat. No. 553288/562085). The contour plot depicting CD4 versus Vα2 TCR expression was derived from gated events with the side and forward light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a FACScan™ system.
Flow cytometric analysis of Vα2 TCR expression on mice peripheral lymphocytes. BALB/c lymph node cells were incubated simultaneously with PE Rat Anti-Mouse CD8b.2 (Cat. No. 553041), PE Rat Anti-Mouse CD4 (Cat. No. 553730/561829/557308) and FITC Rat Anti-Mouse Vα2 TCR (Cat. No. 553288/562085). The contour plot depicting CD4 versus Vα2 TCR expression was derived from gated events with the side and forward light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a FACScan™ system.
Product Details
BD Pharmingen™
T cell receptor alpha, variable region 2
Mouse (QC Testing)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, λ
Soluble αβ TCR from mouse cytotoxic T-cell clone KB5-C20
Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_394759
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
FITC Rat Anti-Mouse Vα2 TCR RUO
Size
25 mg
Cat No.
562085
PE Rat Anti-Mouse CD4 RUO
Size
0.1 mg
Cat No.
553048
PE Rat Anti-Mouse CD4 RUO
Size
0.2 mg
Cat No.
553730
PE Rat Anti-Mouse CD4 RUO
Size
25 mg
Cat No.
561829
553288 Rev. 10
Antibody Details
B20.1
The B20.1 monoclonal antibody specifically binds to most members of the Vα2 T-cell Receptor (TCR) subfamily in mice having the a, b, and c haplotypes of the Tcrb gene complex. B20.1 antibody may crossreact with Vδ8 TCR, which shares >90% sequence homology with Vα2 TCR. Levels of B20.1+ T cells appear to be influenced by Vα haplotypes. Moreover, the frequencies of Vα2+ CD8+ and CD4+ T cells are influenced by H-2 haplotypes.
For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells.
553288 Rev. 10
Format Details
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
494 nm
518 nm
553288 Rev.10
Citations & References
View product citations for antibody "553288" on CiteAb
Development References (3)
-
Grégoire C, Rebaï N, Schweisguth F, et al. Engineered secreted T-cell receptor alpha beta heterodimers.. Proc Natl Acad Sci USA. 1991; 88(18):8077-81. (Clone-specific). View Reference
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Pircher H, Rebaï N, Groettrup M, et al. Preferential positive selection of V alpha 2+ CD8+ T cells in mouse strains expressing both H-2k and T cell receptor V alpha a haplotypes: determination with a V alpha 2-specific monoclonal antibody.. Eur J Immunol. 1992; 22(2):399-404. (Immunogen). View Reference
-
Tomonari K, Fairchild S, Rosenwasser OA. Influence of viral superantigens on V beta- and V alpha-specific positive and negative selection. Immunol Rev. 1993; 131:131-168. (Biology). View Reference
553288 Rev. 10
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.