Skip to main content Skip to navigation
Hamburger Menu
Search icon
Location Selector Location Selector
Norway (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Link Arrow
Solutions

Link Arrow
Discover & Learn

Link Arrow
Resources & Tools

Link Arrow
Support

Link Arrow
Search icon Search icon
Close Menu

      Search functionality is temporarily unavailable due to a system issue. 5:30 p.m - 10:30 p.m CEST on Sunday, January 18, 2026. Read More

      If you encounter problems while ordering, please contact us at BDBCustomerService@bd.com

      Website Feedback
      Alert Icon
      FITC Rat Anti-Mouse CD90.2
      mCD902_FITC_balbC.png

      Flow cytometric analysis of CD90.2 expression on mouse thymocytes.  Thymocytes from an AKR (Left Plot) or BALB/c (Right Plot) mouse were stained with either FITC Rat IgG2a, κ Isotype Control (Cat. No. 553929; dashed line histogram s) or FITC Rat Anti-Mouse CD90.2 antibody (Cat. No. 553003/553004; solid line histograms). The fluorescence histograms showing CD90.2 expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable thymocytes. Flow cytometric analysis was performed using a BD FACSCanto™ System.

      553003Image1.png

      Flow cytometric analysis of CD90.2 expression on mouse thymocytes.  Thymocytes from an AKR (Left Plot) or BALB/c (Right Plot) mouse were stained with either FITC Rat IgG2a, κ Isotype Control (Cat. No. 553929; dashed line histogram s) or FITC Rat Anti-Mouse CD90.2 antibody (Cat. No. 553003/553004; solid line histograms). The fluorescence histograms showing CD90.2 expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable thymocytes. Flow cytometric analysis was performed using a BD FACSCanto™ System.

      mCD902_FITC_balbC.png 553003Image1.png Green-Cap-Blue-Label.png

      Flow cytometric analysis of CD90.2 expression on mouse thymocytes.  Thymocytes from an AKR (Left Plot) or BALB/c (Right Plot) mouse were stained with either FITC Rat IgG2a, κ Isotype Control (Cat. No. 553929; dashed line histogram s) or FITC Rat Anti-Mouse CD90.2 antibody (Cat. No. 553003/553004; solid line histograms). The fluorescence histograms showing CD90.2 expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable thymocytes. Flow cytometric analysis was performed using a BD FACSCanto™ System.

      Flow cytometric analysis of CD90.2 expression on mouse thymocytes.  Thymocytes from an AKR (Left Plot) or BALB/c (Right Plot) mouse were stained with either FITC Rat IgG2a, κ Isotype Control (Cat. No. 553929; dashed line histogram s) or FITC Rat Anti-Mouse CD90.2 antibody (Cat. No. 553003/553004; solid line histograms). The fluorescence histograms showing CD90.2 expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable thymocytes. Flow cytometric analysis was performed using a BD FACSCanto™ System.

      Product Details
      Down Arrow Up Arrow


      BD Pharmingen™
      Thy-1.2; T25; Thymus cell antigen 1, theta
      Mouse (QC Testing)
      Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
      Mouse Thymus / Spleen
      Flow cytometry (Routinely Tested)
      0.5 mg/ml
      AB_394542
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      Show More blue down arrow Show Less blue up arrow
      553004 Rev. 12
      Antibody Details
      Down Arrow Up Arrow
      53-2.1

      The 53-2.1 monoclonal antibody specifically binds to the CD90.2 (Thy-1.2) alloantigen on thymocytes, most peripheral T lymphocytes, some intraepithelial T lymphocytes (IEL, DEC), epithelial cells, fibroblasts, neurons, hematopoietic stem cells, but not B lymphocytes, of most mouse strains. The 53-2.1 antibody has been reported not to crossreact with Thy-1.1 (e.g., AKR/J, PL), or with rat Thy-1. CD90 is a glycophosphatidylinositol-anchored membrane glycoprotein of the Ig superfamily that is involved in signal transduction. In addition, there is evidence that CD90 mediates adhesion of thymocytes to thymic stroma. The 53-2.1 antibody has been reported to block the binding of the Rat Anti-Mouse CD90.2 antibody (Clone 30-H12) to immobilized thymocyte membranes.

      553004 Rev. 12
      Format Details
      Down Arrow Up Arrow
      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      FITC
      494 nm
      518 nm
      553004 Rev.12
      Citations & References
      Down Arrow Up Arrow
      View product citations for antibody "553004" on CiteAb

      Development References (13)

      1. Borrello MA, Phipps RP. Differential Thy-1 expression by splenic fibroblasts defines functionally distinct subsets. Cell Immunol. 1996; 173(2):198-206. (Biology). View Reference
      2. He HT, Naquet P, Caillol D, Pierres M. Thy-1 supports adhesion of mouse thymocytes to thymic epithelial cells through a Ca2(+)-independent mechanism. J Exp Med. 1991; 173(2):515-518. (Biology). View Reference
      3. Hueber AO, Raposo G, Pierres M, He HT. Thy-1 triggers mouse thymocyte apoptosis through a bcl-2-resistant mechanism. J Exp Med. 1994; 179(3):785-796. (Biology). View Reference
      4. Ikuta K, Uchida N, Friedman J, Weissman IL. Lymphocyte development from stem cells. Annu Rev Immunol. 1992; 10:759-783. (Biology). View Reference
      5. Kroczek RA, Gunter KC, Germain RN, Shevach EM. Thy-1 functions as a signal transduction molecule in T lymphocytes and transfected B lymphocytes. Nature. 1986; 322(6075):181-184. (Biology). View Reference
      6. LeFrancois L. Extrathymic differentiation of intraepithelial lymphocytes: generation of a separate and unequal T-cell repertoire. Immunol Today. 1991; 12(12):436-438. (Biology). View Reference
      7. Ledbetter JA, Herzenberg LA. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979; 47:63-90. (Immunogen: Blocking). View Reference
      8. Ledbetter JA, Rouse RV, Micklem HS, Herzenberg LA. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med. 1980; 152(2):280-295. (Biology). View Reference
      9. Radrizzani M, Carminatti H, Pivetta OH, Idoyaga Vargas VP. Developmental regulation of Thy 1.2 rate of synthesis in the mouse cerebellum. J Neurosci Res. 1995; 42(2):220-227. (Biology). View Reference
      10. Tigelaar RE, Lewis JM, Bergstresser PR. TCR gamma/delta+ dendritic epidermal T cells as constituents of skin-associated lymphoid tissue. J Invest Dermatol. 1990; 94(6):58S-63S. (Biology). View Reference
      11. Williams AF, Gagnon J. Neuronal cell Thy-1 glycoprotein: homology with immunoglobulin. Science. 1982; 216(4547):696-703. (Biology). View Reference
      12. Zheng B, Han S, Kelsoe G. T helper cells in murine germinal centers are antigen-specific emigrants that downregulate Thy-1. J Exp Med. 1996; 184(3):1083-1091. (Biology). View Reference
      13. Zhong RK, Donnenberg AD, Edison L, Harrison DE. The appearance of Thy-1- donor T cells in the peripheral circulation 3-6 weeks after bone marrow transplantation suggests an extrathymic origin. Int Immunol. 1996; 8(2):171-176. (Biology). View Reference
      View All (13) View Less
      553004 Rev. 12

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.