FITC Mouse Anti-Human CD1b

This SKU will be discontinuing Apr 2024. For additional support, contact your local applications specialist. Contact Us

BD Pharmingen™ FITC Mouse Anti-Human CD1b

Name: FITC Mouse Anti-Human CD1b
Brand: BD Pharmingen™
SKU: 555969

Clone M-T101

(RUO)

View all Formats

Protocol Library

Scientific Resources

Profile of MOLT-4 cells analyzed on a FACScan (BDIS, San Jose, CA)

Spectrum Viewer

Protocol Library

Scientific Resources

Product Details

Brand: BD Pharmingen™

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human Thymus Cells

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 20 µl

Workshop Number: IV T077

RRID: AB_2073397

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Companion Products

FITC Mouse IgG1, κ Isotype Control RUO

Size 100 Tests Cat No. 555748

555969 Rev. 5

Antibody Details

M-T101

The M-T101 monoclonal antibody specifically binds to CD1b, a 45 kDa membrane glycoprotein expressed in association with β2-microglobulin. CD1b is expressed on cortical thymocytes (moderately), Langerhans cells and interdigitating cells. CD1b has also been reported to react with pyramidal cells in brain. CD1b may participate in a "non-classical" antigen presentation pathway.

555969 Rev. 5

Format Details

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: FITC

Excitation Source: Blue 488 nm

Excitation Max: 494 nm

Emission Max: 518 nm

555969 Rev.5

Citations & References

Development References (3)

Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.

555969 Rev. 5

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.