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557400Image1.png

Flow cytometric analysis of CD11c expression on mouse splenocytes. Spleen NK cells were stained with PE Mouse Anti-Mouse NK-1.1 (Cat. No. 557391/553165) and either FITC Hamster IgG Isotype Control (Cat. No. 553953; left panel) or FITC Hamster anti-Mouse CD11c antibody (Cat. No. 553801/557400; right panel). The contour plots were derived from events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD FACSCan™ flow cytometry system.

09704A_557400_image2.png

Flow cytometric analysis of CD11c expression on mouse splenocytes. Spleen NK cells were stained with PE Mouse Anti-Mouse NK-1.1 (Cat. No. 557391/553165) and either FITC Hamster IgG Isotype Control (Cat. No. 553953; left panel) or FITC Hamster anti-Mouse CD11c antibody (Cat. No. 553801/557400; right panel). The contour plots were derived from events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD FACSCan™ flow cytometry system.

557400Image1.png 09704A_557400_image2.png 340352_01.png Green-Cap-Blue-Label.png

Flow cytometric analysis of CD11c expression on mouse splenocytes. Spleen NK cells were stained with PE Mouse Anti-Mouse NK-1.1 (Cat. No. 557391/553165) and either FITC Hamster IgG Isotype Control (Cat. No. 553953; left panel) or FITC Hamster anti-Mouse CD11c antibody (Cat. No. 553801/557400; right panel). The contour plots were derived from events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD FACSCan™ flow cytometry system.

Flow cytometric analysis of CD11c expression on mouse splenocytes. Spleen NK cells were stained with PE Mouse Anti-Mouse NK-1.1 (Cat. No. 557391/553165) and either FITC Hamster IgG Isotype Control (Cat. No. 553953; left panel) or FITC Hamster anti-Mouse CD11c antibody (Cat. No. 553801/557400; right panel). The contour plots were derived from events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD FACSCan™ flow cytometry system.

Product Details
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BD Pharmingen™
Cd11c; Itgax; Integrin alpha-X; Integrin αX; Cr4; Complement receptor 4
Mouse (QC Testing)
Armenian Hamster IgG1, λ2
C57BL/6 Mouse Intestinal Intraepithelial Lymphocytes
Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_396683
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
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Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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553801 Rev. 16
Antibody Details
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HL3

The HL3 monoclonal antibody specifically binds to the integrin αx chain of gp150, 95 (CD11c/CD18). CD11c is expressed on dendritic cells, CD4- CD8+ intestinal intraepithelial lymphocytes (IEL) and some NK cells. It is upregulated on IEL and lymph-node T cells following in vivo activation. Cells of the monocyte/macrophage lineage have been reported to express low levels of CD11c. CD11c plays a role in binding of iC3b.

553801 Rev. 16
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
494 nm
518 nm
553801 Rev.16
Citations & References
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View product citations for antibody "553801" on CiteAb

Development References (8)

  1. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
  2. Burt BM, Plitas G, Stableford JA, et al. CD11c identifies a subset of murine liver natural killer cells that responds to adenoviral hepatitis. J Leukoc Biol. 2008; 84(4):1039-1046. (Clone-specific). View Reference
  3. Fagarasan S, Muramatsu M, Suzuki K, Nagaoka H, Hiai H, Honjo T. Critical roles of activation-induced cytidine deaminase in the homeostasis of gut flora. Science. 2002; 298(5597):1424-1427. (Clone-specific: Immunofluorescence). View Reference
  4. Gao JX, Liu X, Wen J, et al. Differentiation of monocytic cell clones into CD8 alpha+ dendritic cells (DC) suggests that monocytes can be direct precursors for both CD8 alpha+ and CD8 alpha- DC in the mouse. J Immunol. 2003; 170(12):5927-5935. (Biology). View Reference
  5. Huleatt JW, Lefrançois L. Antigen-driven induction of CD11c on intestinal intraepithelial lymphocytes and CD8+ T cells in vivo.. J Immunol. 1995; 154(11):5684-93. (Immunogen: Immunoprecipitation). View Reference
  6. Larson RS, Springer TA. Structure and function of leukocyte integrins. Immunol Rev. 1990; 114:181-217. (Biology). View Reference
  7. Maraskovsky E, Brasel K, Teepe M, et al. Dramatic increase in the numbers of functionally mature dendritic cells in Flt3 ligand-treated mice: multiple dendritic cell subpopulations identified. J Exp Med. 1996; 184(5):1953-1962. (Biology). View Reference
  8. Pulendran B, Lingappa J, Kennedy MK, et al. Developmental pathways of dendritic cells in vivo: distinct function, phenotype, and localization of dendritic cell subsets in FLT3 ligand-treated mice. J Immunol. 1997; 159(5):2222-2231. (Clone-specific: Immunohistochemistry). View Reference
View All (8) View Less
553801 Rev. 16

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.