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BV421 Rat Anti-Mouse IL-23 Receptor
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BV421 Rat Anti-Mouse IL-23 Receptor
Flow cytometric analysis using BD OptiBuild™ BV421 Rat Anti-Mouse IL-23 Receptor antibody (Cat. No. 744368; solid line histogram) on IL-23 Receptor-transfected cells, with Isotype Control (dotted line histogram). Flow cytometry was performed using a BD LSRFortessa™ X-20  Flow Cytometer System.
Flow cytometric analysis using BD OptiBuild™ BV421 Rat Anti-Mouse IL-23 Receptor antibody (Cat. No. 744368; solid line histogram) on IL-23 Receptor-transfected cells, with Isotype Control (dotted line histogram). Flow cytometry was performed using a BD LSRFortessa™ X-20  Flow Cytometer System.
Product Details
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BD OptiBuild™
Il23r; IL-23R; Interleukin 23 receptor
Mouse (Tested in Development)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
Mouse IL-23 Receptor Recombinant Protein
Flow cytometry (Qualified)
0.2 mg/ml
209590
AB_2742183
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  7. Researchers should determine the optimal concentration of this reagent for their individual applications.
  8. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  10. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  11. Pacific Blue™ is a trademark of Life Technologies Corporation.
744368 Rev. 3
Antibody Details
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O78-1208

The O78-1208 monoclonal antibody specifically binds to the mouse Interleukin-23 Receptor (IL-23R) subunit that is encoded by the il23r gene. The IL-23R subunit is a type I transmembrane glycoprotein and member of the hemopoietin receptor superfamily. The mouse IL-23 Receptor complex is comprised of IL-23R and IL-12 receptor beta 1 (IL-12Rβ1) subunits. The IL-23R complex can bind IL-23, a cytokine that plays roles in innate and adaptive immunity as well as in autoimmune diseases, eg, by the generation and maintenance of Th17 cells. Mouse IL-23R is expressed by activated/memory CD4+ T cells, Th1, Th2 and Th17 cells, γδ T cells, dendritic cells and macrophages as determined by IL-23R mRNA expression and IL-23R-GFP reporter mouse studies. The IL-23-bound IL-23R  complex transduces an intracellular signal pathway mediated by a Jak-STAT signaling cascade.

744368 Rev. 3
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
744368 Rev.3
Citations & References
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View product citations for antibody "744368" on CiteAb

Development References (6)

  1. Awasthi A, Riol-Blanco L, Jager A, et al. Cutting edge: IL-23 receptor gfp reporter mice reveal distinct populations of IL-17-producing cells. J Immunol. 2009; 182(10):5904-5908. (Biology). View Reference
  2. Jones LL, Alli R, Li B, Geiger TL. Differential T Cell Cytokine Receptivity and Not Signal Quality Distinguishes IL-6 and IL-10 Signaling during Th17 Differentiation.. J Immunol. 2016; 196(7):2973-85. (Clone-specific: Flow cytometry). View Reference
  3. Lankford CS, Frucht DM. A unique role for IL-23 in promoting cellular immunity. J Leukoc Biol. 2003; 73(1):49-56. (Biology). View Reference
  4. Monk JM, Hou TY, Turk HF, McMurray DN, Chapkin RS. n3 PUFAs reduce mouse CD4+ T-cell ex vivo polarization into Th17 cells.. J Nutr. 2013; 143(9):1501-8. (Clone-specific: Flow cytometry). View Reference
  5. Parham C, Chirica M, Timans J, et al. A receptor for the heterodimeric cytokine IL-23 is composed of IL-12Rbeta1 and a novel cytokine receptor subunit, IL-23R. J Immunol. 2002; 168(11):5699-5708. (Biology: Calcium Flux). View Reference
  6. Petermann F, Rothhammer V, Claussen MC, et al. gammadelta T cells enhance autoimmunity by restraining regulatory T cell responses via an interleukin-23-dependent mechanism. Immunity. 2010; 33(3):351-363. (Biology). View Reference
View All (6) View Less
744368 Rev. 3

 

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.