Product Details
BD Pharmingen™
Mouse IgG1, κ
Intracellular staining (flow cytometry) (Routinely Tested), Bioimaging, Immunofluorescence (Tested During Development), Western blot (Not Recommended)
0.5 mg/ml
AB_2739144
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Triton is a trademark of the Dow Chemical Company.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
Fixation Buffer RUO
Size
100 ML
Cat No.
554655
Perm Buffer III RUO
Size
125 ML
Cat No.
558050
Biotin Mouse IgG1 κ Isotype Control RUO
Size
100 Tests
Cat No.
555747
APC Streptavidin RUO
Size
0.1 mg
Cat No.
554067
DAPI Solution RUO
Size
1 mg
Cat No.
564907
565271 Rev. 1
Antibody Details
1A12-6-18
Monoclonal antibody 1A12-6-18 reacts with Green Fluorescent Protein (GFP), which is a 27 kDa bioluminescent protein first purified from the jellyfish, Aequorea victoria. GFP exhibits green fluorescence when exposed to blue or ultraviolet light. Additionally, GFP can be introduced into the genome of and expressed in a wide variety of cell types, making it a useful reporter for tracking gene expression or protein localization. A number of GFP mutants have been developed with varying fluorescence intensity and spectra. Monoclonal antibody 1A12-6-18 is known to react with AcGFP, and is not reactive towards ZsGreen1.
565271 Rev. 1
Format Details
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
565271 Rev.1
Citations & References
View product citations for antibody "565271" on CiteAb
Development References (4)
-
Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC. Green fluorescent protein as a marker for gene expression. Science. 1994; 263(5148):802-805. (Biology). View Reference
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Heinen AP, Wanke F, Moos S, et al. Improved Method to Retain Cytosolic Reporter Protein Fluorescence While Staining for Nuclear Proteins. Cytometry A. 2014; 85A:621-627. (Biology). View Reference
-
Kain SR, Adams M, Kondepudi A, Yang TT, Ward WW, Kitts P. Green fluorescent protein as a reporter of gene expression and protein localization. Biotechniques. 1995; 19(4):650-655. (Biology). View Reference
-
Nybo K. GFP Imaging in Fixed Cells. Biotechniques. 2012; 52(6):359-360. (Biology). View Reference
565271 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.