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FITC Mouse Anti-Human HLA-DQ
Product Details
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BD™
MHC class II HLA-DQ; MHC HLA-DQ
Human
Mouse IgG1, κ
Tonsillar B lymphocytes (non-E-rosetting cells)
Flow cytometry
25 μg/mL
20 μL
AB_400304
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO (GMP)


Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

347453 Rev. 1
Antibody Details
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SK10

The Anti–HLA-DQ antibody, clone SK10, is derived from the hybridization of NS-1 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with tonsillar B lymphocytes (non–E-rosetting cells).

Anti-HLA-DQ (Leu-10) recognizes a class II major histocompatibility complex (MHC) antigen, Mr 26 to 34 kdaltons (kDa), distinct from HLA-DR and HLA-DP antigens. Anti-HLA-DQ recognizes a common polymorphic epitope present on HLA-DQ molecules of cells expressing DQw1 and DQw3 (usually associated with DR1, DR2, DR4, DR5, DRw8, DRw9, and DRw10) and absent from DQw2 (usually associated with DR3, and DR7). Therefore, Anti-HLA-DQ will not react with cells from DR3 or DR 7 homozygotes or DR3/7 heterozygotes.

347453 Rev. 1
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
347453 Rev.1
Citations & References
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View product citations for antibody "347453" on CiteAb

Development References (8)

  1. Brodsky FM. A matrix approach to human class II histocompatibility antigens: reactions of four monoclonal antibodies with the products of nine haplotypes.. Immunogenetics. 1984; 19(3):179-94. (Biology). View Reference
  2. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  3. Chen YX, Evans RL, Pollack MS, et al. Characterization and expression of the HLA-DC antigens defined by anti-Leu-10. Hum Immunol. 1984; 10:221-235. (Biology).
  4. Clinical and Laboratory Standards Institute. 2005. (Biology).
  5. Crumpton MF, Bodmer JG, Bodmer WF, Heyes JM, Lindsay J, Rudd CE. Biochemistry of class II antigens: workshop report. In: :29.
  6. Edwards JA, Durant BM, Jones DB, Evans PR, Smith JL. Differential expression of HLA class II antigens in fetal human spleen: relationship of HLA-DP, DQ, and DR to immunoglobulin expression.. J Immunol. 1986; 137(2):490-7. (Biology). View Reference
  7. Gonwa TH, Frost JP, Karr RW. All human monocytes have the capability of expressing HLA-DQ and HLA-DP molecules upon stimulation with interferon-γ. J Immunol. 1986; 137:519-524. (Biology).
  8. Robbins PA, Evans EL, Ding AH, Warner NL, Brodsky FM. Monoclonal antibodies that distinguish between class II antigens (HLA-DP, DQ, and DR) in 14 haplotypes.. Hum Immunol. 1987; 18(4):301-13. (Biology). View Reference
View All (8) View Less
347453 Rev. 1

 

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