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Product Details
BD™
SPN; Sialophorin; Galactoglycoprotein/GALGP; GPL115; LEUK; Leukosialin; LSN
Human
Mouse IgG1, κ
Flow cytometry
50 µg/mL
5 μL
VI N-L166
AB_2870374
RUO (GMP)
655407 Rev. 1
Antibody Details
1G10
The CD43 antibody, clone 1G10, is derived from the hybridization of X63 mouse myeloma cells with spleen cells isolated from mice immunized with a lymph node suspension from a patient with Hodgkin lymphoma.
The CD43 antibody reacts specifically with the major 95–135-kilodalton (kDa) sialoglycoprotein found on most human leucocytes. The CD43 antigen is also known as leukosialin or sialophorin.
655407 Rev. 1
Format Details
APC-H7
The BD Horizon™ APC-H7 dye is a part of the BD APC red family of dyes. This tandem fluorochrome is comprised of a Allophycocyanin (APC) donor that has excitation maxima (Ex Max) of 659 nm and an acceptor dye, H7, with an emission maximum (Em Max) at 782 nm. APC-H7, driven by BD innovation, is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 780 nm (e.g., a 760/60 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
APC-H7
659 nm
782 nm
655407 Rev.1
Citations & References
View product citations for antibody "655407" on CiteAb
Development References (13)
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Ardman B, Sikorski MA, Staunton DE. CD43 interferes with T-lymphocyte adhesion. Proc Natl Acad Sci U S A. 1992; 89:5001-5005. (Biology).
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Bazil V, Strominger JL. CD43, the major sialoglycoprotein of human leukocytes, is proteolytically cleaved from the surface of stimulated lymphocytes and granulocytes. Proc Natl Acad Sci U S A. 1993 May; 90(9):3792-3796. (Biology). View Reference
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Bettaieb A, Farace F, Mitjavila MT, et al. Use of a monoclonal antibody (GA3) to demonstrate lineage restricted O-glycosylation on leukosialin during terminal erythroid differentiation. Blood. 1988; 71:1226-1233. (Biology).
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Borche L, Lozano F, Vilella R, Vives J. CD43 monoclonal antibodies recognize the large sialoglycoprotein of human leukocytes. Eur J Immunol. 1987; 17(10):1523-1526. (Biology). View Reference
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Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
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Clinical and Laboratory Standards Institute. 2005. (Biology).
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Horejsi V, Stockinger H. CD43 Workshop Panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:494-497.
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Howell DN, Ahuja V, Jones L, Blow O, Sanfilippo FP. Differential expression of CD43 (leukosialin, sialophorin) by mononuclear phagocyte populations. J Leukoc Biol. 1994; 55:536-544. (Biology).
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Matsumoto M, Shigeta A, Miyasaka M, Hirata T. CD43 plays both antiadhesive and proadhesive roles in neutrophil rolling in a context-dependent manner. J Immunol. 2008; 181:3628-3635. (Biology).
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Nathan C, Xie QW, Halbwachs-Mecarelli L, Jin WW. Albumin inhibits neutrophil spreading and hydrogen peroxide release by blocking the shedding of CD43 (sialophorin, leukosialin). J Cell Biol. 1993; 90:3792-3796. (Biology).
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Urano-Tashiro Y, Yajima A, Takashima E, Takahashi Y, Konishi K. Binding of the Streptococcus gordonii DL1 surface protein Hsa to the host cell membrane glycoproteins CD11b, CD43, and CD50. Infect Immun. 2008; 76:4686-4691. (Biology).
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Zola H, Swart B, Nicholson I, Voss E. Leukocyte and Stromal Cell Molecules: The CD Markers. 2007. (Biology).
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van den Berg TK, Nath D, Ziltener HJ, et al. Cutting edge: CD43 functions as a T cell counterreceptor for the macrophage adhesion receptor sialoadhesin (Siglec-1). J Immunol. (Biology).
655407 Rev. 1
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
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