Skip to main content Skip to navigation
Purified Rat Anti-Human IL-7
Product Details
Down Arrow Up Arrow

BD Pharmingen™
Human (QC Testing)
Rat IgG1, κ
Recombinant Human IL-7
ELISA Capture (Routinely Tested), Neutralization, Western blot (Tested During Development)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

ELISA Capture: The purified BVD10-40F6 antibody (Cat.No. 554493) is useful as a capture antibody for a sandwich ELISA for measuring human IL-7 protein levels.  Purified BVD10-40F6 antibody can be paired with the biotinylated BVD10-11C10 antibody (Cat. No. 554494) as the detection antibody, with recombinant human IL-7 (Cat. No. 554608) as the standard. Purified BVD10-40F6 antibody should be titrated 1-4 µg/ml to determine optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of human IL-7 ranging from ~2,000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology please visit the protocols sections or the chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site,

Note 1: This ELISA pair shows no cross-reactivity with any of the cytokines tested (eg, mouse IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 p70, IL-15, GM-CSF, IFN-γ, MCP-1, TCA-3, TNF; human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-γ, lymphotactin, MCP-1, MCP-2, MIP-1α, MIP-1β, NT-3, PDGF-AA, sCD23 , SCF, TNF, LT-α, VEGF; rat IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, TNF).

Note 2: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems.

Neutralization: The no azide/low endotoxin format (NA/LE) of BVD10-40F6 antibody, Cat. No. 554492, is recommended for neutralization of human IL-7 bioactivity. A suitable NA/LE rat IgG1 isotype control to match the BVD10-40F6 antibody is the R3-34 antibody, Cat. No, 559072.

Western Blot: The BVD10-40F6 antibody (Cat. No. 554493) has been found useful for Western blotting. Please note that this application is not routinely tested at BD Biosciences Pharmingen

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
554493 Rev. 1
Antibody Details
Down Arrow Up Arrow

The BVD10-40F6 antibody reacts with human interleukin-7 (IL-7).  The immunogen used to generate the BVD10-40F6 hybridoma was recombinant human IL-7. This is a neutralizing antibody.

554493 Rev. 1
Format Details
Down Arrow Up Arrow
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
554493 Rev.1
Citations & References
Down Arrow Up Arrow
View product citations for antibody "554493" on CiteAb

Development References (2)

  1. Abrams J. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001; 1:6.20-6.21. (Clone-specific: ELISA). View Reference
  2. Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Clone-specific: ELISA, Neutralization). View Reference
554493 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.