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Purified Rat Anti-Human IL-3
Product Details
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BD Pharmingen™
Human (QC Testing)
Rat IgG1
Recombinant Human IL-3
ELISA Capture (Routinely Tested), Neutralization, Western blot (Tested During Development)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

ELISA Capture: The purified BVD8-3G11 antibody (Cat. No. 554672) is useful as a capture antibody in a sandwich ELISA for measuring human IL-3 protein levels. Purified BVD8-3G11 antibody can be paired with the biotinylated BVD3-1F9 antibody (Cat. #554674) as the detecting antibody, with recombinant human IL-3 (Cat. No. 554604) as the standard. This purified capture antibody should be pretitrated in the range of 1 - 4 µg/ml to determine its optimal concentration for ELISA. To obtain linear standard curves, doubling dilutions of human IL-3 ranging from ~2,000 to 15 pg/ml should be used. For specific methodology, please visit our web site,, and go to the protocols section or the chapter on ELISA in the Immune Function Handbook.

Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not

recommended for assay of serum samples. For testing human IL-3 in complex biological fluids such as serum or plasma, our BD OptEIA™

Human IL-3 ELISA Set (Cat. No. 555228) is recommended.

WB: The BVD8-3G11 antibody has been found useful for western blotting. A concentration of 1-5 µg/ml (in conjunction with the AKP-conjugated goat anti-rat Igs has been found to enable visualization of ≤ 100 ng/lane of recombinant IL-3 (e.g., Cat. No. 554604), under reducing conditions.

Neutralization: The BVD8-3G11 antibody (Cat. No. 554671) is useful for neutralization of human IL-3 bioactivity.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
554672 Rev. 1
Antibody Details
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The BVD8-3G11 antibody reacts with human interleukin-3 (IL-3). The immunogen used to generate the BVD8-3G11 hybridoma was yeast-expressed recombinant human IL-3. This is a neutralizing antibody.

554672 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
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Citations & References
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View product citations for antibody "554672" on CiteAb

Development References (4)

  1. Abrams J. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001; 1:6.20-6.21. (Clone-specific: ELISA). View Reference
  2. Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Clone-specific: ELISA, Neutralization). View Reference
  3. Abrams JS, Silver JE, Van Dyke RE, Gleich GI. Eosinophil-active cytokines in human disease: development and use of monoclonal antibodies to IL-3, IL-5 and GMCSF. In: Gleich GJ and Kay AB, ed. Eosinophils in Allergy and Inflammation. New York: Dekker; 1994:133-157.
  4. Kaushansky K, Shoemaker SG, Broudy VC. Structure-function relationships of interleukin-3. An analysis based on the function and binding characteristics of a series of interspecies chimera of gibbon and murine interleukin-3. J Clin Invest. 1992; 90(5):1879-1888. (Clone-specific: ELISA). View Reference
View All (4) View Less
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Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.