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Purified NA/LE Mouse Anti-Human IL-8
Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG1, κ
Recombinant human IL-8
ELISA (Routinely Tested), Neutralization (Tested During Development), Western blot (Reported)
1.0 mg/ml
AB_395531
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
554726 Rev. 5
Antibody Details
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2A2

The 2A2 antibody reacts with both the 72 and 77 amino acid forms of human IL-8. The immunogen used to produce the 2A2 hybridoma was E. coli-expressed recombinant human IL-8 (77 amino acid form).

This antibody has been reported to show no cross-reactivity with the following cytokines or chemokines:  Human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-12 (p40 or p70), IL-13, IL-15, eotaxin, GRO, IFN- γ, MCP-1, MCP-2, MCP-3, MIP-1α, MIP-1β, NAP-2, PF4, RANTES, SCF, TNF, LT-α.

Neutralization: Investigators are advised that this material is not routinely tested for the Neutralization application and are highly encouraged to both titrate this material and include appropriate controls in relevant experiments.  This antibody has previously been reported to be useful for the neutralization of recombinant human IL-8 when measured with a calcium flux assay using 250 ng/mL recombinant human IL-8 (Cat. No. 554609) to stimulate calcium mobilization in 1x10^7 cells/mL human lysed whole blood as indicator cells.  Preincubation of the antibody with recombinant human IL-8 can neutralize calcium mobilization with the following representative ranges:

50% Neutralization (ND50) at 8 - 10 μg/mL

> 95% Neutralization at 150 - 500 μg/mL

554726 Rev. 5
Format Details
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NA/LE
NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
NA/LE
554726 Rev.5
Citations & References
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Development References (2)

  1. Leonard EJ, Sylvester I, Yoshimura T, et al. Current Protocols in Immunology. John Wiley and Sons; 1995:6.12.1-6.12.26.
  2. Matsushima K, Oppenheim JJ. Interleukin 8 and MCAF: novel inflammatory cytokines inducible by IL 1 and TNF. Cytokine. 1989; 1(1):2-13. (Clone-specific). View Reference
554726 Rev. 5

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

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