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Purified Mouse Anti-Mouse IgG1[a]
Product Details
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BD Pharmingen™
Igh-4a; Ighg1; IgG1
Mouse SJL IgG2a, κ
Not reported
ELISA (Routinely Tested)
0.5 mg/ml
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Indirect ELISA: Purified mouse IgG1 (a allotype) [MN 557273] may be used as the antigen (e.g coating plates). Purified anti-mouse IgG1[a] allotype specific antibody [MN 553499] may be used at ~ 2 µg/ml followed by biotinylated anti-mouse IgG2a [b] allotype specific antibody [MN 553504] at ~ 2 µg/ml and avidin-HRP [MN 554058] for detection.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to for technical protocols.
553499 Rev. 10
Antibody Details
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This antibody is specific for mouse IgG1 of the Igh-C[a] and the c, d, e, f, g, h, n, o, and p haplotypes (e.g. BALB/c, C58, A, AKR, CBA, C3H, DBA/1, DBA/2, NZB, SWR). It has been reported not to react with IgG1 of the Igh-C[b] haplotype (e.g. C57BL/6, C57BL/10, SJL).

553499 Rev. 10
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
553499 Rev.10
Citations & References
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View product citations for antibody "553499" on CiteAb

Development References (2)

  1. Huang CM, Huang HJ, Lee SC. Detection of immunoglobulin heavy chain IgG3 polymorphism in wild mice with xenogeneic monoclonal antibodies. Immunogenetics. 1984; 20(5):565-575. (Biology). View Reference
  2. Parsons M, Oi VT, Huang CM, Herzenberg LA. Structural characterization of mouse immunoglobulin allotypic determinants (allotopes) defined by monoclonal antibodies. Immunogenetics. 1983; 18(4):323-334. (Biology). View Reference
553499 Rev. 10

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.