Biotin Mouse Anti-Rat IgA

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BD Pharmingen™ Biotin Mouse Anti-Rat IgA

Name: Biotin Mouse Anti-Rat IgA
Brand: BD Pharmingen™
SKU: 553912

Clone A93-2

(RUO)

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Product Details

Brand: BD Pharmingen™

Reactivity: Rat (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Pooled rat IgA

Application: ELISA Detection (Routinely Tested)

Concentration: 0.5 mg/ml

RRID: AB_395130

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

ELISA: For the rat IgA sandwich ELISA, biotinylated A93-2 mAb is optimal for detection when used with purified A93-3 mAb (Cat. no. 553913) for capture and and the purified rat IgA, clone R3-30, (Cat. No. 553945) as the protein standard.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

553912 Rev. 6

Antibody Details

A93-2

The A93-2 antibody reacts specifically with rat IgA. It does not react with other Ig isotypes.

553912 Rev. 6

Format Details

Biotin

Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.

Format: Biotin

553912 Rev.6

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.