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Biotin Mouse Anti-Human IL-2
Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG1, κ
Recombinant Human IL-2 protein
ELISA Detection (Routinely Tested)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Recommended Assay Procedures

ELISA Detection:  Biotinylated B33-2 antibody (Cat. No. 555040) is useful as a detection antibody in sandwich ELISA for measuring human IL-2 protein levels. The biotinylated B33-2 antibody can be paired with purified MQ1-17H12 antibody (Cat. No. 554563) as the capture antibody and with recombinant human IL-2 (Cat. No. 554603) as the standard. Biotinylated B33-2 antibody should be titrated (0.5 - 2.0 µg/mL) to determine the optimal concentration for ELISA detection. To obtain linear standard curves, doubling dilutions of human IL-2 ranging from ~2,000 to 15 pg/mL are recommended for inclusion in each ELISA plate.  This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems.  For serum or plasma, the BD OptEIA™ Human IL-2 ELISA Set (Cat. No. 555190) or BD OptEIA™ Human IL-2 ELISA Kit II (Cat. No.550611) are recommended.

This ELISA pair has been reported to show no cross-reactivity with the following cytokines: mouse IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL- 10, IL-12p70, IL-15, GM-CSF, IFN-γ, MCP-1, TCA-3, TNF; human IL-1α, IL-1β, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-γ, lymphotactin, MCP-1, MCP-2 MIP-1α, MIP-1β, NT-3, PDGF-AA, sCD23 , SCF, TNF, LT-α, VEGF; rat IL- 2, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, TNF).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. This product is manufactured and sold under license from Pestka Biomedical Laboratories, Inc. (d/b/a PBL InterferonSource) and may be used solely as indicated. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics is strictly prohibited. This product is covered by U.S. Patent No. 5,597,901 and Bulgarian Patent No. BG1895.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to for technical protocols.
555040 Rev. 2
Antibody Details
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The B33-2 antibody reacts with human interleukin-2 (IL-2). The immunogen used to generate the B33-2 hybridoma was affinity-purified, recombinant human IL-2 protein.

555040 Rev. 2
Format Details
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Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
555040 Rev.2
Citations & References
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Development References (1)

  1. Abrams J. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001; 1:6.20-6.21. (Biology). View Reference
555040 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.