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Biotin Human anti-Mouse/Rat TNF
Biotin Human anti-Mouse/Rat TNF

Mouse TNF sandwich ELISA curve: The curve was generated by sandwich ELISA using the purified TN3-19.12 (Cat. No. 557516) as capture antibody, doubling dilutions of recombinant mouse TNF protein (Cat. No. 554589) and biotin 516D1A1 as detection antibody. Streptavidin-HRP (Cat. No. 554066) plus TMB substrate (Cat. No. 555214) were used to develop the ELISA.

Mouse TNF sandwich ELISA curve: The curve was generated by sandwich ELISA using the purified TN3-19.12 (Cat. No. 557516) as capture antibody, doubling dilutions of recombinant mouse TNF protein (Cat. No. 554589) and biotin 516D1A1 as detection antibody. Streptavidin-HRP (Cat. No. 554066) plus TMB substrate (Cat. No. 555214) were used to develop the ELISA.

Product Details
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BD Pharmingen™
Mouse (QC Testing), Rat (Reported)
Human Ig, κ
Mouse TNF
ELISA (Routinely Tested)
0.5 mg/ml
AB_647175
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Product Notices

  1. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
558415 Rev. 1
Antibody Details
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516D1A1

The 516D1A1 Fab antibody reacts with mouse and rat tumor-necrosis factor (TNF, formerly known as TNF-α) proteins. The 516D1A1 Fab was selected by screening a human Fab phage display library (Hoet et al.) licensed from Dyax Corp (Cambridge, MA) with recombinant mouse TNF protein. The biology and function of the TNF protein has been extensively reviewed (Hehlgans et al.) in the literature.

The biotinylated 516D1A1 antibody is useful as a detection antibody for a sandwich ELISA that measures mouse and rat TNF protein levels. Purified Hamster Anti-Mouse/Rat TNF (Cat. No.557516) as capture antibody can be paired with biotinylated 516D1A1 as the detection antibody, using purified recombinant mouse TNF protein (Cat. No. 554589) or purified recombinant rat TNF protein (Cat. No. 555109) as standard. The biotinylated 516D1A1 antibody should be titrated between 0.25 - 2.0 µg/ml to determine its optimal ELISA detection concentration. To obtain linear standard curves, doubling dilutions of purified mouse or rat TNF protein ranging from 31.25 pg/ml to 2,000 pg/ml are recommended for inclusion in each ELISA plate. Investigators may find methodology information to be useful in Techniques for Immune Function Analysis Application Handbook 1st Edition. BD Biosciences (2003)-- Chapter 7:  ELISA for specifically measuring the levels of cytokines, chemokines, and inflammatory mediators and their receptors.  For this ELISA antibody pair, no cross-reactivity has been observed with the following: Rat IL-1α, IL-2, IL-4, IL-6, IL-10, IL-18, GM-CSF, IFN-γ; mouse IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL-17, IL-18, M-CSF, GM-CSF, IFN-γ, MCP-1, MIP-1α, MIP-1β, sTNFRI, sTNFRII, RANTES; human TNF.

558415 Rev. 1
Format Details
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
558415 Rev.1
Citations & References
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Development References (2)

  1. Hehlgans T, Pfeffer K. The intriguing biology of the tumour necrosis factor/tumour necrosisfactor receptor superfamily: players, rules and the games. Immunology. 2005; 115(1):1-20. (Biology). View Reference
  2. Hoet RM, Cohen EH, Kent RB, et al. Generation of high-affinity human antibodies by combining donor-derived and synthetic complementarity-determining-region diversity. Nat Biotechnol. 2005; 23(3):344-348. (Clone-specific: ELISA). View Reference
558415 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

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