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Galectin-3 Expression 1. Surface _ PEC: Thioglycolate-elicited C57BL/6 mouse peritoneal exudate cells (PEC) were preincubated with Mouse BD Fc Block™ (Cat. No. 553141/553142) and stained with either Purified Rat IgG2a, κ Isotype Control (Cat. No. 553927; Dotted Line Histogram) or Purified Rat Anti-Galectin-3 (Cat. No. 567905; Solid Line Histogram) at 0.06 µg/test followed by staining with PE Goat Anti-Rat IgG (Cat. No. 550767). DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The histogram shows Galectin-3 expression (or Ig Isotype control staining) derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. 2. Surface + Intracellular _ PEC: Thioglycolate-elicited PEC were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), and permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723). The cells were stained with either Purified Rat IgG2a, κ Isotype Control (Dotted Line Histogram) or Purified Rat Anti-Galectin-3 (Solid Line Histogram) at 0.06 µg/test, followed by staining with PE Goat Anti-Rat IgG. Total cellular Galectin-3 expression (or Ig Isotype control staining) by intact PEC is shown. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer and FlowJo™ software. Data shown are not lot specific. 3. Immunohistofluorescence analysis of Galectin-3 expression in mouse intestine. Following antigen retrieval with BD Pharmingen™ Retrievagen A Buffer (Cat. No. 550524), a formalin-fixed, paraffin-embedded BALB/c mouse intestine section was stained with Purified Rat Anti-Galectin-3 (Cat. No. 567904) followed by BD Horizon™ BV480 Goat Anti-Rat Ig (Cat. No. 564878, Left Image; Pseudo-colored Green). Sections were counterstained was with DAPI (Cat. No. 564907; Middle Image; Pseudo-colored Blue) as a nuclear stain. The images were captured and merged (Right Image) using a standard epifluorescence microscope system equipped with a 20× objective.
BD Pharmingen™ Purified Rat Anti-Galectin-3
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Preparation And Storage
Product Notices
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Companion Products
The M3/38 monoclonal antibody specifically recognizes Galectin-3 (Gal-3 or gal3) which is also known as Galactose-specific lectin 3, Mac-2, MAC2, and Carbohydrate-binding protein 35 (CBP 35). Galectin-3 is an ~30-35 kDa protein that includes an N-terminal proline-rich tandem repeat domain as well as a C-terminal region with one carbohydrate recognition domain (CRDs). Galectin-3 is encoded by Lgals3 (lectin, galactose binding, soluble 3) which belongs to the galectin family. It is expressed by a variety of cells including activated macrophages, dendritic cells (DCs), granulocytes, mast cells, and epithelial cells. Galectin-3 can be detected in monomeric or multimeric forms in cytoplasmic, nuclear, cell surface and extracellular locations. Galectin-3 is a β-galactoside-binding lectin that has multiple intracellular and extracellular functions implicated in biological processes including RNA splicing as well as cellular chemoattraction and adhesion, activation, growth, proliferation, differentiation, and apoptosis. Upregulated Galectin-3 expression is implicated in inflammation and disease states including cancer progression and metastasis.
Development References (8)
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Demotte N, Wieërs G, Van Der Smissen P, et al. A galectin-3 ligand corrects the impaired function of human CD4 and CD8 tumor-infiltrating lymphocytes and favors tumor rejection in mice.. Cancer Res. 2010; 70(19):7476-88. (Clone-specific: Flow cytometry). View Reference
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Dong R, Zhang M, Hu Q, et al. Galectin-3 as a novel biomarker for disease diagnosis and a target for therapy (Review).. Int J Mol Med. 2018; 41(2):599-614. (Biology). View Reference
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Flotte TJ, Springer TA, Thorbecke GJ. Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets. Am J Pathol. 1983; 111(1):112-124. (Clone-specific: Immunohistochemistry). View Reference
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Gray RM, Davis MJ, Ruby KM, Voss PG, Patterson RJ, Wang JL. Distinct effects on splicing of two monoclonal antibodies directed against the amino-terminal domain of galectin-3.. Arch Biochem Biophys. 2008; 475(2):100-8. (Clone-specific: Functional assay, Western blot). View Reference
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Ho MK, Springer TA. Mac-2, a novel 32,000 Mr mouse macrophage subpopulation-specific antigen defined by monoclonal antibodies.. J Immunol. 1982; 128(3):1221-8. (Immunogen: Flow cytometry, Immunoprecipitation, Radioimmunoassay). View Reference
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Johannes L, Jacob R, Leffler H. Galectins at a glance.. J Cell Sci. 2018; 131(9):jcs208884. (Biology). View Reference
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Karlsson A, Follin P, Leffler H, Dahlgren C. Galectin-3 activates the NADPH-oxidase in exudated but not peripheral blood neutrophils.. Blood. 1998; 91(9):3430-8. (Clone-specific: Flow cytometry). View Reference
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Newlaczyl AU, Yu LG. Galectin-3--a jack-of-all-trades in cancer.. Cancer Lett. 2011; 313(2):123-8. (Biology). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.