PE Mouse Anti-Human Cβ1 TCR
Multicolor flow cytometric analysis of TCR Cβ1 expression by human peripheral blood lymphocytes. Human peripheral blood was treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes. After washing, the cells were stained with either APC Mouse Anti-Human CD3 (Left Plots; Cat. No. 555335/561810/561811), BD Horizon™ BV421 Mouse Anti-Human CD4 (Middle Plots; Cat. No. 562424/562425), or APC Mouse Anti-Human CD8 (Right Plots; Cat. No. 555369/561421/561952/561953) antibody and either PE Mouse IgG2a, κ Isotype Control (Cat. No. 554648; Top Plots) or PE Mouse Anti-Human TCR Cβ1 antibody (Cat. No. 565776; Bottom Plots) at 1 µg/test. Two-color flow cytometric contour plots showing the correlated expression of CD3, CD4 or CD8 versus TCR Cβ1 (or Ig Isotype Control staining) were derived from gated events with the forward and side light-scatter characteristics of viable human peripheral blood lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
BD Pharmingen™ PE Mouse Anti-Human Cβ1 TCR
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