FITC Mouse Anti-Human CD75

BD Pharmingen™ FITC Mouse Anti-Human CD75

Name: FITC Mouse Anti-Human CD75
Brand: FITC Mouse Anti-Human CD75
SKU: 555654

Clone LN1 (RUO)

Profile of peripheral blood lymphocytes analyzed on a FACScan (BDIS, San Jose, CA)

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Product Details

Brand: BD Pharmingen™

Alternative Name: CDw75

Reactivity: Human (QC Testing)

Isotype: Mouse IgM, κ

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 20 µl

Workshop Number: V CD75.3, BP162

RRID: AB_396009

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Companion Products

FITC Mouse IgM, κ Isotype Control RUO

Size 100 Tests Cat No. 555583

555654 Rev. 6

Antibody Details

LN1

Reacts with CD75, a carbohydrate determinant generated by the action of intracellular α2,6 sialyltransferase, expressed on mature B cells. In the lymph node, strong reactivity has been detected on germinal center B cells and weaker expression observed in mantle zone B cells. Evidence suggests that CD75 plays a role in intercellular adhesion and is a ligand for CD22.

555654 Rev. 6

Format Details

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: FITC

Excitation Source: Blue 488 nm

Excitation Max: 494 nm

Emission Max: 518 nm

555654 Rev.6

Citations & References

View product citations for antibody "555654" on CiteAb

Development References (2)

Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
Stamenkovic I, Sgroi D, Aruffo A, Sy MS, Anderson T. The B lymphocyte adhesion molecule CD22 interacts with leukocyte common antigen CD45RO on T cells and alpha 2-6 sialyltransferase, CD75, on B cells. Cell. 1991; 66(6):1133-1144. (Biology). View Reference

555654 Rev. 6

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.