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      FITC Mouse Anti-Human CD15
      FITC Mouse Anti-Human CD15
      Flow cytometric analysis of CD15 expression on human peripheral blood granulocytes. Whole blood was stained with FITC Mouse Anti-Human CD15 antibody (Cat. No. 562370; solid line histogram) or with a FITC Mouse IgG1, κ Isotype Control (Cat. No. 555748; dotted line histogram). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable granulocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
      FITC Mouse Anti-Human CD15
      Flow cytometric analysis of CD15 expression on human peripheral blood granulocytes. Whole blood was stained with FITC Mouse Anti-Human CD15 antibody (Cat. No. 562370; solid line histogram) or with a FITC Mouse IgG1, κ Isotype Control (Cat. No. 555748; dotted line histogram). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable granulocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
      Product Details
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      BD Pharmingen™
      3-fucosyl-N-acetyllactosamine; 3-FAL
      Human (QC Testing)
      Mouse IgG1, κ
      Flow cytometry (Routinely Tested)
      5 µl
      2526
      AB_11153493
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      562370 Rev. 1
      Antibody Details
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      W6D3

      The W6D3 monoclonal antibody specifically binds to 3-fucosyl-N-acetyllactosamine (3-FAL), a 220 kDa carbohydrate structure, also called X-hapten. 3-FAL is expressed on >95% of granulocytes, including neutrophils and eosinophils, and on monocytes to a varying degree, but not on lymphocytes or basophils. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis. Most CD15 antibodies are IgM isotype; clone W6D3 is a mouse IgG1 isotype. In comparison studies with clone HI98, a known CD15 antibody, clone W6D3 shows brighter fluorescence staining and its binding can be blocked by clone HI98.

      562370 Rev. 1
      Format Details
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      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      FITC
      Blue 488 nm
      494 nm
      518 nm
      562370 Rev.1
      Citations & References
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      View product citations for antibody "562370" on CiteAb

      Development References (3)

      1. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
      2. Lund-Johansen F, Olweus J, Horejsi V, et al. Activation of human phagocytes through carbohydrate antigens (CD15, sialyl-CD15, CDw17, and CDw65).. J Immunol. 1992; 148(10):3221-9. (Biology). View Reference
      3. Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007.
      562370 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.