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      BUV395 Mouse Anti-Human IL-22
      BUV395 Mouse Anti-Human IL-22
      Multicolor flow cytometric analysis of IL-22 expression in human peripheral blood mononuclear cells (PBMCs). PBMCs were cultured in complete tissue culture medium with Phorbol 12-Myristate 13-Acetate (PMA; Sigma, 50 ng/ml) and Ionomycin (Sigma, 500 ng/ml) in the presence of BD GolgiPlug™ Protein Transport Inhibitor (Cat. No. 555029) for 6 hours. The cells were harvested, then fixed and permeabilized using BD Cytofix™ Fixation Buffer (Cat. No. 554655) and BD Perm/Wash™ buffer (Cat. No. 554723). The cells were then stained with PE Mouse Anti-Human CD4 (Cat. No. 555347/561843/561844) and either BD Horizon™ BUV395 Mouse IgG2a, κ Isotype Control (Cat. No. 563809; Left Panel) or BD Horizon™ BUV395 Mouse Anti-IL-Human 22 antibody (Cat. No. 567783/567784; Right Panel) at 0.06 µg/test. Bivariate pseudocolor density plots showing the correlated expression of IL-22 (or Ig Isotype control staining) versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      BUV395 Mouse Anti-Human IL-22
      Multicolor flow cytometric analysis of IL-22 expression in human peripheral blood mononuclear cells (PBMCs). PBMCs were cultured in complete tissue culture medium with Phorbol 12-Myristate 13-Acetate (PMA; Sigma, 50 ng/ml) and Ionomycin (Sigma, 500 ng/ml) in the presence of BD GolgiPlug™ Protein Transport Inhibitor (Cat. No. 555029) for 6 hours. The cells were harvested, then fixed and permeabilized using BD Cytofix™ Fixation Buffer (Cat. No. 554655) and BD Perm/Wash™ buffer (Cat. No. 554723). The cells were then stained with PE Mouse Anti-Human CD4 (Cat. No. 555347/561843/561844) and either BD Horizon™ BUV395 Mouse IgG2a, κ Isotype Control (Cat. No. 563809; Left Panel) or BD Horizon™ BUV395 Mouse Anti-IL-Human 22 antibody (Cat. No. 567783/567784; Right Panel) at 0.06 µg/test. Bivariate pseudocolor density plots showing the correlated expression of IL-22 (or Ig Isotype control staining) versus CD4 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      Product Details
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      BD Horizon™
      interleukin-22; IL-10-related T-cell-derived inducible factor; IL-TIF; ILTIF; IL-22a, ilTIFa; Iltif; TIFa
      Human (QC Testing), Mouse (Tested in Development)
      Mouse BALB/c IgG2a, κ
      Human IL-22 Recombinant Protein
      Intracellular staining (flow cytometry) (Routinely Tested)
      0.2 mg/ml
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome-conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads. This will ensure that BD® CompBeads are appropriate for your specific cellular application.

      For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant™ Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant™ Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant™ Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant™ Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant™ Stain Buffer Plus (Cat. No. 566385).

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. An isotype control should be used at the same concentration as the antibody of interest.
      6. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
      7. BD Horizon Brilliant Ultraviolet 395 is covered by one or more of the following US patents: 8,158,444; 8,575,303; 8,354,239.
      8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
      9. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
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      Antibody Details
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      MH22B2

      The MH22B2 monoclonal antibody specifically recognizes human Interleukin-22 (IL-22), which is encoded by the IL22 gene. Cross-reactivity of MH22B2 mAb to mouse IL-22 (which shares 79% amino acid identity with human IL-22) has been observed by ELISA and by flow cytometry of HEK293 cells transfected with mouse IL-22 and TH17-differentiated CD4-positive T cells from C57BL/6 mice. IL-22 (with or without other cytokines) is secreted by many T-cell and innate-lymphoid-cell populations. Evidence that IL-22 plays a key role in mucosal immunity includes the restricted expression of the alpha subunit of the heterodimeric IL-22 receptor, called IL-22R1, on epithelial cells and cells of epithelial origin. At epithelial surfaces, IL-22 elicits antimicrobial defenses and maintains epithelial integrity. Alternatively, uncontrolled IL-22 production can result in certain inflammatory disorders. Regulation of IL-22 expression is complex, involving other cytokines (eg, IL-6, IL-23, and TGF-β) and many transcription factors, (eg, AHR, c-Maf, STAT3, RORɤT, BATF, and others).

      The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. With an Ex Max near 348 nm and an Em Max near 395 nm, BD Horizon™ BUV395 can be excited by the ultraviolet laser (355 nm) laser and detected with a 379/28 filter. This dye has been exclusively developed by BD Biosciences as an optimal dye for use on instruments equipped with the ultraviolet laser and has virtually no spillover into any other detector.

      Format Details
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      BUV395
      The BD Horizon Brilliant™ Ultraviolet 395 (BUV395) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This base dye is a polymer fluorochrome with an excitation maximum (Ex Max) of 348-nm and an emission maximum (Em Max) at 395-nm. Driven by BD innovation, BUV395 is designed to be excited by the ultraviolet laser (355-nm) and detected using an optical filter centered near 380-nm (e.g., 379/28-nm bandpass filter). BUV395 is the ideal dye when using only one detector on the ultraviolet laser as it spills into no other detectors and no other fluors spill into it. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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      BUV395
      Ultraviolet 355 nm
      348 nm
      395 nm
      Citations & References
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      Development References (8)

      1. Colonna M. Interleukin-22-producing natural killer cells and lymphoid tissue inducer-like cells in mucosal immunity.. Immunity. 2009; 31(1):15-23. (Biology). View Reference
      2. Le-Thi-Phuong T, Dumoutier L, Renauld JC, Van Snick J, Coutelier JP. Divergent roles of IFNs in the sensitization to endotoxin shock by lactate dehydrogenase-elevating virus.. Int Immunol. 2007; 19(11):1303-11. (Clone-specific: ELISA). View Reference
      3. Martin B, Hirota K, Cua DJ, Stockinger B, Veldhoen M. Interleukin-17-producing gammadelta T cells selectively expand in response to pathogen products and environmental signals.. Immunity. 2009; 31(2):321-30. (Clone-specific: Flow cytometry). View Reference
      4. Rutz S, Eidenschenk C, Ouyang W. IL-22, not simply a Th17 cytokine.. Immunol Rev. 2013; 252(1):116-32. (Biology). View Reference
      5. Suurmond J, Habets KL, Dorjée AL, Huizinga TW, Toes RE. Expansion of Th17 Cells by Human Mast Cells Is Driven by Inflammasome-Independent IL-1β. J Immunol. 2016; 164(11):4473-4481. (Biology). View Reference
      6. Trifari S, Kaplan CD, Tran EH, Crellin NK, Spits H. Identification of a human helper T cell population that has abundant production of interleukin 22 and is distinct from T(H)-17, T(H)1 and T(H)2 cells.. Nat Immunol. 2009; 10(8):864-71. (Biology). View Reference
      7. Veldhoen M, Hirota K, Westendorf AM, et al. The aryl hydrocarbon receptor links TH17-cell-mediated autoimmunity to environmental toxins.. Nature. 2008; 453(7191):106-9. (Immunogen: Flow cytometry). View Reference
      8. Zenewicz LA. IL-22: There Is a Gap in Our Knowledge.. Immunohorizons. 2018; 2(6):198-207. (Biology). View Reference
      View All (8) View Less

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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