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      Biotin Rat Anti-Mouse Vβ 6 T-Cell Receptor
      01362C_553192_image2.png

      Two-color analysis of the expression of Vβ 6 TCR on peripheral T lymphocytes. C57BL/6 lymph nodes cells were incubated simultaneously with Biotin Rat Anti-Mouse Vβ 6 T-Cell Receptor (Cat. No. 553192; right panel), PE Rat Anti-Mouse CD4 (Cat. No. 553048/553049), and PE Rat Anti-Mouse CD8a (Cat. No. 553032/553033) monoclonal antibodies, followed by Avidin FITC (Cat. No. 554057). The fluorescence contour plots were derived from gated events based on the forward and side light-scattering of viable lymphocytes. Flow cytometry was performed on a FACScan™.

      553192Image1.png

      Two-color analysis of the expression of Vβ 6 TCR on peripheral T lymphocytes. C57BL/6 lymph nodes cells were incubated simultaneously with Biotin Rat Anti-Mouse Vβ 6 T-Cell Receptor (Cat. No. 553192; right panel), PE Rat Anti-Mouse CD4 (Cat. No. 553048/553049), and PE Rat Anti-Mouse CD8a (Cat. No. 553032/553033) monoclonal antibodies, followed by Avidin FITC (Cat. No. 554057). The fluorescence contour plots were derived from gated events based on the forward and side light-scattering of viable lymphocytes. Flow cytometry was performed on a FACScan™.

      01362C_553192_image2.png 553192Image1.png clear-clear-white.jpg

      Two-color analysis of the expression of Vβ 6 TCR on peripheral T lymphocytes. C57BL/6 lymph nodes cells were incubated simultaneously with Biotin Rat Anti-Mouse Vβ 6 T-Cell Receptor (Cat. No. 553192; right panel), PE Rat Anti-Mouse CD4 (Cat. No. 553048/553049), and PE Rat Anti-Mouse CD8a (Cat. No. 553032/553033) monoclonal antibodies, followed by Avidin FITC (Cat. No. 554057). The fluorescence contour plots were derived from gated events based on the forward and side light-scattering of viable lymphocytes. Flow cytometry was performed on a FACScan™.

      Two-color analysis of the expression of Vβ 6 TCR on peripheral T lymphocytes. C57BL/6 lymph nodes cells were incubated simultaneously with Biotin Rat Anti-Mouse Vβ 6 T-Cell Receptor (Cat. No. 553192; right panel), PE Rat Anti-Mouse CD4 (Cat. No. 553048/553049), and PE Rat Anti-Mouse CD8a (Cat. No. 553032/553033) monoclonal antibodies, followed by Avidin FITC (Cat. No. 554057). The fluorescence contour plots were derived from gated events based on the forward and side light-scattering of viable lymphocytes. Flow cytometry was performed on a FACScan™.

      Product Details
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      BD Pharmingen™
      TCR V beta 6; TCR Vβ6
      Mouse (QC Testing)
      Rat F344, also known as Fischer, CDF IgG2b, λ
      C57BL/6 Mouse Helper T-Cell Clone OI11
      Flow cytometry (Routinely Tested)
      0.5 mg/ml
      AB_394699
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      553192 Rev. 12
      Antibody Details
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      RR4-7

      The RR4-7 antibody specifically reacts with the Vβ 6 T-Cell Receptor (TCR) of mice having the a (e.g., C57BR, C57L, SJL) and b (e.g., A, BALB/c, CBA/Ca, C3H/He, C57BL, DBA/1) haplotypes of the Tcrb gene complex. The Tcrb-V6 gene locus is deleted in mice having the c (e.g., RIII) haplotype. Vβ 6 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigen encoded by Mtv-7 (Mls-1[a], Mls[a]) endogenous provirus (e.g., AKR, CBA/J, C58, DBA/2, NZB), or Mtv-43 endogenous provirus (e.g., MA/MyJ). Exogenous MMTV-SW, as well as endogenous Mtv-44-encoded superantigen (e.g., NZW), also causes incomplete elimination of Vβ 6 TCR-expressing T cells. Plate-bound RR4-7 antibody activates Vβ 6 TCR-bearing T cells, soluble RR4-7 mAb blocks in vitro proliferation and cytolytic activities of Vβ 6 TCR-bearing T-cell clones, and injection of the antibody results in in vivo depletion of Vβ 6 TCR-bearing T cells.

      553192 Rev. 12
      Format Details
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      Biotin
      Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
      Biotin
      553192 Rev.12
      Citations & References
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      View product citations for antibody "553192" on CiteAb

      Development References (14)

      1. Fairchild S, Rosenwasser OA, Dyson PJ, Tomonari K. Tcrb-V3+ T-cell deletion and a new mouse mammary tumor provirus, Mtv-44. Immunogenetics. 1992; 36(3):189-194. (Biology). View Reference
      2. Haqqi TM, Banerjee S, Anderson GD, David CS. RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes. J Exp Med. 1989; 169(6):1903-1909. (Biology). View Reference
      3. Held W, Shakhov AN, Waanders G, et al. An exogenous mouse mammary tumor virus with properties of Mls-1a (Mtv-7). J Exp Med. 1992; 175(6):1623-1633. (Biology). View Reference
      4. Jones LA, Chin LT, Longo DL, Kruisbeek AM. Peripheral clonal elimination of functional T cells. Science. 1990; 250(4988):1726-1729. (Biology). View Reference
      5. Jones LA, Chin LT, Merriam GR, Nelson LM, Kruisbeck AM. Failure of clonal deletion in neonatally thymectomized mice: tolerance is preserved through clonal anergy. J Exp Med. 1990; 172(5):1277-1285. (Biology). View Reference
      6. Kanagawa O, Palmer E, Bill J. The T cell receptor V beta 6 domain imparts reactivity to the Mls-1a antigen. Cell Immunol. 1989; 119(2):412-426. (Immunogen: Blocking). View Reference
      7. Kanagawa O. In vivo T cell tumor therapy with monoclonal antibody directed to the V beta chain of T cell antigen receptor. J Exp Med. 1989; 170(5):1513-1519. (Clone-specific: Depletion). View Reference
      8. Kruisbeek AM, Shevach EM. Proliferative assays for T cell function. Curr Protoc Immunol. 2004; 3:3.12.1-3.12.14. (Clone-specific: Activation). View Reference
      9. Ramsdell F, Lantz T, Fowlkes BJ. A nondeletional mechanism of thymic self tolerance. Science. 1989; 246(4933):1038-1041. (Biology). View Reference
      10. Rocha B, Vassalli P, Guy-Grand D. The V beta repertoire of mouse gut homodimeric alpha CD8+ intraepithelial T cell receptor alpha/beta + lymphocytes reveals a major extrathymic pathway of T cell differentiation. J Exp Med. 1991; 173(2):483-486. (Biology). View Reference
      11. Rudy CK, Kraus E, Palmer E, Huber BT. Mls-1-like superantigen in the MA/MyJ mouse is encoded by a new mammary tumor provirus that is distinct from Mtv-7. J Exp Med. 1992; 175(6):1613-1621. (Biology). View Reference
      12. Tomonari K, Fairchild S. Positive and negative selection of Tcrb-V6+ T cells. Immunogenetics. 1992; 36(4):230-237. (Biology). View Reference
      13. Utsunomiya Y, Kosaka H, Kanagawa O. Differential reactivity of V beta 9 T cells to minor lymphocyte stimulating antigen in vitro and in vivo. Eur J Immunol. 1991; 21(4):1007-1011. (Clone-specific: Activation). View Reference
      14. Webb S, Morris C, Sprent J. Extrathymic tolerance of mature T cells: clonal elimination as a consequence of immunity. Cell. 1990; 63(6):1249-1256. (Biology). View Reference
      View All (14) View Less
      553192 Rev. 12

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      For Research Use Only. Not for use in diagnostic or therapeutic procedures.