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BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Mouse Siglec-G
Clone SH1 (RUO)
Multicolor flow cytometric analysis of Siglec-G expression on BALB/c splenocytes. Splenic leucocytes were stained with Alexa Fluor® 647 Mouse Anti-Mouse Siglec-G (Cat. No. 563336) and Alexa Fluor® 700 Rat Anti-Mouse CD3 Molecular Complex (Cat. No. 561388) antibodies. A two-color flow cytometric dot plot showing the correlated expression patterns of CD3 versus Siglec-G was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes (Left Panel). Splenic leucocytes were similarly stained with Alexa Fluor® 647 Mouse Anti-Mouse Siglec-G and FITC Rat Anti-Mouse CD45R/B220 (Cat. No. 553088/553087/561877) antibodies. A two-color flow cytometric dot plot shows the correlated expression patterns of CD45R/B220 versus Siglec-G for viable lymphocytes (Right Panel). Flow cytometry was performed using a BD LSR™ II Flow Cytometer System.
Multicolor flow cytometric analysis of Siglec-G expression on BALB/c splenocytes. Splenic leucocytes were stained with Alexa Fluor® 647 Mouse Anti-Mouse Siglec-G (Cat. No. 563336) and Alexa Fluor® 700 Rat Anti-Mouse CD3 Molecular Complex (Cat. No. 561388) antibodies. A two-color flow cytometric dot plot showing the correlated expression patterns of CD3 versus Siglec-G was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes (Left Panel). Splenic leucocytes were similarly stained with Alexa Fluor® 647 Mouse Anti-Mouse Siglec-G and FITC Rat Anti-Mouse CD45R/B220 (Cat. No. 553088/553087/561877) antibodies. A two-color flow cytometric dot plot shows the correlated expression patterns of CD45R/B220 versus Siglec-G for viable lymphocytes (Right Panel). Flow cytometry was performed using a BD LSR™ II Flow Cytometer System.
Multicolor flow cytometric analysis of Siglec-G expression on BALB/c splenocytes. Splenic leucocytes were stained with Alexa Fluor® 647 Mouse Anti-Mouse Siglec-G (Cat. No. 563336) and Alexa Fluor® 700 Rat Anti-Mouse CD3 Molecular Complex (Cat. No. 561388) antibodies. A two-color flow cytometric dot plot showing the correlated expression patterns of CD3 versus Siglec-G was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes (Left Panel). Splenic leucocytes were similarly stained with Alexa Fluor® 647 Mouse Anti-Mouse Siglec-G and FITC Rat Anti-Mouse CD45R/B220 (Cat. No. 553088/553087/561877) antibodies. A two-color flow cytometric dot plot shows the correlated expression patterns of CD45R/B220 versus Siglec-G for viable lymphocytes (Right Panel). Flow cytometry was performed using a BD LSR™ II Flow Cytometer System.
Multicolor flow cytometric analysis of Siglec-G expression on BALB/c splenocytes. Splenic leucocytes were stained with Alexa Fluor® 647 Mouse Anti-Mouse Siglec-G (Cat. No. 563336) and Alexa Fluor® 700 Rat Anti-Mouse CD3 Molecular Complex (Cat. No. 561388) antibodies. A two-color flow cytometric dot plot showing the correlated expression patterns of CD3 versus Siglec-G was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes (Left Panel). Splenic leucocytes were similarly stained with Alexa Fluor® 647 Mouse Anti-Mouse Siglec-G and FITC Rat Anti-Mouse CD45R/B220 (Cat. No. 553088/553087/561877) antibodies. A two-color flow cytometric dot plot shows the correlated expression patterns of CD45R/B220 versus Siglec-G for viable lymphocytes (Right Panel). Flow cytometry was performed using a BD LSR™ II Flow Cytometer System.
Multicolor flow cytometric analysis of Siglec-G expression on BALB/c splenocytes. Splenic leucocytes were stained with Alexa Fluor® 647 Mouse Anti-Mouse Siglec-G (Cat. No. 563336) and Alexa Fluor® 700 Rat Anti-Mouse CD3 Molecular Complex (Cat. No. 561388) antibodies. A two-color flow cytometric dot plot showing the correlated expression patterns of CD3 versus Siglec-G was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes (Left Panel). Splenic leucocytes were similarly stained with Alexa Fluor® 647 Mouse Anti-Mouse Siglec-G and FITC Rat Anti-Mouse CD45R/B220 (Cat. No. 553088/553087/561877) antibodies. A two-color flow cytometric dot plot shows the correlated expression patterns of CD45R/B220 versus Siglec-G for viable lymphocytes (Right Panel). Flow cytometry was performed using a BD LSR™ II Flow Cytometer System.
Multicolor flow cytometric analysis of Siglec-G expression on BALB/c splenocytes. Splenic leucocytes were stained with Alexa Fluor® 647 Mouse Anti-Mouse Siglec-G (Cat. No. 563336) and Alexa Fluor® 700 Rat Anti-Mouse CD3 Molecular Complex (Cat. No. 561388) antibodies. A two-color flow cytometric dot plot showing the correlated expression patterns of CD3 versus Siglec-G was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes (Left Panel). Splenic leucocytes were similarly stained with Alexa Fluor® 647 Mouse Anti-Mouse Siglec-G and FITC Rat Anti-Mouse CD45R/B220 (Cat. No. 553088/553087/561877) antibodies. A two-color flow cytometric dot plot shows the correlated expression patterns of CD45R/B220 versus Siglec-G for viable lymphocytes (Right Panel). Flow cytometry was performed using a BD LSR™ II Flow Cytometer System.
Product Details
BD Pharmingen™
Siglecg; mSiglec-G; Siglec10; Siglec 10; CD330
Mouse (QC Testing)
Mouse IgG1, κ
Mouse Extracellular Siglec-G Recombinant Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2738142
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Alexa Fluor® 647 Mouse IgG1 κ Isotype Control RUO
Size
100 Tests
Cat No.
557714
FITC Rat Anti-Mouse CD45R/B220 RUO
Size
0.5 mg
Cat No.
553088
FITC Rat Anti-Mouse CD45R/B220 RUO
Size
0.1 mg
Cat No.
553087
FITC Rat Anti-Mouse CD45R/B220 RUO
Size
25 mg
Cat No.
561877
Alexa Fluor® 700 Rat Anti-Mouse CD3 Molecular Complex RUO
Size
50 µg
Cat No.
561388
563336 Rev. 1
Antibody Details
SH1
The SH1 monoclonal antibody specifically binds to mouse Siglec-G (sialic acid binding immunoglobulin-like lectin G), also known as Siglec10. Siglec-G is a Type I transmembrane glycoprotein that belongs to the Immunoglobulin superfamily. Siglec-G functions as an adhesion molecule that mediates sialic-acid dependent binding to cells. Siglec-G is expressed on cells of the B cell lineage. Siglec-G is most highly expressed by pre-B cells and B1a cells within the B cell lineage and is not detectable on T cells. Siglec-G can inhibit B cell receptor-mediated calcium signaling when it is overexpressed. Mice that lack Siglec-G have significantly increased numbers of B1a cells that begins early in development and is B cell intrinsic. Siglec-G-deficient mice have higher titers of natural IgM antibodies than their normal counterparts. Mouse Siglec-G is the ortholog of human Siglec-10 (also known as, CD330).
563336 Rev. 1
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
653 nm
669 nm
563336 Rev.1
Citations & References
View product citations for antibody "563336" on CiteAb
Development References (4)
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Aizawa H, Zimmermann N, Carrigan PE, Lee JJ, Rothenberg ME, Bochner BS. Molecular analysis of human Siglec-8 orthologs relevant to mouse eosinophils: identification of mouse orthologs of Siglec-5 (mSiglec-F) and Siglec-10 (mSiglec-G). Genomics. 2003; 82(5):521-530. (Biology). View Reference
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Hoffmann A, Kerr S, Jellusova J, et al. Siglec-G is a B1 cell-inhibitory receptor that controls expansion and calcium signaling of the B1 cell population. Nat Immunol. 2007; 8(7):695-704. (Biology). View Reference
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Jellusova J, Düber S, Gückel E, et al. Siglec-G regulates B1 cell survival and selection. J Immunol. 2010; 185(6):3277-3284. (Biology). View Reference
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Nitschke L. CD22 and Siglec-G: B-cell inhibitory receptors with distinct functions. Immunol Rev. 2009; 230(1):128-143. (Biology). View Reference
563336 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.