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BD Pharmingen™ Alexa Fluor® 488 Rat Anti-Mouse F4/80-Like Receptor
Clone 6F12 (RUO)
Three-color flow cytometric analysis of F4/80-Like Receptor expression on mouse bone marrow cells. Mouse bone-marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with APC Rat Anti-Mouse CD11b (Cat. No. 553312/561690) and PE Hamster Anti-Mouse CD11c (Cat. No. 553802/557401/561044) antibodies and either Alexa Fluor® 488 Rat IgG2a, κ Isotype Control (Cat. No. 557676, Left Panels) or Alexa Fluor® 488 Rat Anti-Mouse F4/80-Like Receptor antibody (Cat. No. 564227; Right Panels). Two-color flow cytometric dot plots showing the correlated expression patterns of CD11b (Upper Panels) or CD11c (Lower Panels) versus F4/80-Like Receptor (or Ig Isotype control staining) were generated from gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Three-color flow cytometric analysis of F4/80-Like Receptor expression on mouse bone marrow cells. Mouse bone-marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with APC Rat Anti-Mouse CD11b (Cat. No. 553312/561690) and PE Hamster Anti-Mouse CD11c (Cat. No. 553802/557401/561044) antibodies and either Alexa Fluor® 488 Rat IgG2a, κ Isotype Control (Cat. No. 557676, Left Panels) or Alexa Fluor® 488 Rat Anti-Mouse F4/80-Like Receptor antibody (Cat. No. 564227; Right Panels). Two-color flow cytometric dot plots showing the correlated expression patterns of CD11b (Upper Panels) or CD11c (Lower Panels) versus F4/80-Like Receptor (or Ig Isotype control staining) were generated from gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Immunohistofluorescent analysis of F4/80-Like Receptor expression by cells within C57BL/6 mouse spleen. A mouse spleen cryosection (5 µm) was fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), blocked with 5% goat serum and 1% BSA diluted in 1x PBS, and stained with Alexa Fluor® 488 Rat Anti-Mouse F4/80-Like Receptor antibody (Cat. No. 564227, pseudo-colored red), BD Horizon™ BV480 Rat Anti-Mouse CD3 Molecular Complex antibody (Cat. No. 565642, pseudo-colored green), and Alexa Fluor® 647 Rat Anti-Mouse CD45R/B220 (Cat. No. 557683, pseudo-colored blue). Slides were mounted with ProLong® Gold and the images were captured on a standard epifluorescence microscope. Original magnification, 20x.
Immunohistofluorescent analysis of F4/80-Like Receptor expression by cells within C57BL/6 mouse spleen. A mouse spleen cryosection (5 µm) was fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), blocked with 5% goat serum and 1% BSA diluted in 1x PBS, and stained with Alexa Fluor® 488 Rat Anti-Mouse F4/80-Like Receptor antibody (Cat. No. 564227, pseudo-colored red), BD Horizon™ BV480 Rat Anti-Mouse CD3 Molecular Complex antibody (Cat. No. 565642, pseudo-colored green), and Alexa Fluor® 647 Rat Anti-Mouse CD45R/B220 (Cat. No. 557683, pseudo-colored blue). Slides were mounted with ProLong® Gold and the images were captured on a standard epifluorescence microscope. Original magnification, 20x.
Three-color flow cytometric analysis of F4/80-Like Receptor expression on mouse bone marrow cells. Mouse bone-marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with APC Rat Anti-Mouse CD11b (Cat. No. 553312/561690) and PE Hamster Anti-Mouse CD11c (Cat. No. 553802/557401/561044) antibodies and either Alexa Fluor® 488 Rat IgG2a, κ Isotype Control (Cat. No. 557676, Left Panels) or Alexa Fluor® 488 Rat Anti-Mouse F4/80-Like Receptor antibody (Cat. No. 564227; Right Panels). Two-color flow cytometric dot plots showing the correlated expression patterns of CD11b (Upper Panels) or CD11c (Lower Panels) versus F4/80-Like Receptor (or Ig Isotype control staining) were generated from gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Three-color flow cytometric analysis of F4/80-Like Receptor expression on mouse bone marrow cells. Mouse bone-marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with APC Rat Anti-Mouse CD11b (Cat. No. 553312/561690) and PE Hamster Anti-Mouse CD11c (Cat. No. 553802/557401/561044) antibodies and either Alexa Fluor® 488 Rat IgG2a, κ Isotype Control (Cat. No. 557676, Left Panels) or Alexa Fluor® 488 Rat Anti-Mouse F4/80-Like Receptor antibody (Cat. No. 564227; Right Panels). Two-color flow cytometric dot plots showing the correlated expression patterns of CD11b (Upper Panels) or CD11c (Lower Panels) versus F4/80-Like Receptor (or Ig Isotype control staining) were generated from gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Immunohistofluorescent analysis of F4/80-Like Receptor expression by cells within C57BL/6 mouse spleen. A mouse spleen cryosection (5 µm) was fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), blocked with 5% goat serum and 1% BSA diluted in 1x PBS, and stained with Alexa Fluor® 488 Rat Anti-Mouse F4/80-Like Receptor antibody (Cat. No. 564227, pseudo-colored red), BD Horizon™ BV480 Rat Anti-Mouse CD3 Molecular Complex antibody (Cat. No. 565642, pseudo-colored green), and Alexa Fluor® 647 Rat Anti-Mouse CD45R/B220 (Cat. No. 557683, pseudo-colored blue). Slides were mounted with ProLong® Gold and the images were captured on a standard epifluorescence microscope. Original magnification, 20x.
Immunohistofluorescent analysis of F4/80-Like Receptor expression by cells within C57BL/6 mouse spleen. A mouse spleen cryosection (5 µm) was fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), blocked with 5% goat serum and 1% BSA diluted in 1x PBS, and stained with Alexa Fluor® 488 Rat Anti-Mouse F4/80-Like Receptor antibody (Cat. No. 564227, pseudo-colored red), BD Horizon™ BV480 Rat Anti-Mouse CD3 Molecular Complex antibody (Cat. No. 565642, pseudo-colored green), and Alexa Fluor® 647 Rat Anti-Mouse CD45R/B220 (Cat. No. 557683, pseudo-colored blue). Slides were mounted with ProLong® Gold and the images were captured on a standard epifluorescence microscope. Original magnification, 20x.
Product Details
BD Pharmingen™
Fire; Emr4; EGF-like module receptor 4; D17Ertd479e; Egf-tm7
Mouse (QC Testing)
Rat IgG2a, κ
CHO cells expressing recombinant FIRE fusion protein
Flow cytometry (Routinely Tested), Immunofluorescence (Tested During Development)
0.2 mg/ml
AB_2738682
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
Size
0.5 mg
Cat No.
553142
Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
Size
0.1 mg
Cat No.
553141
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
PE Hamster Anti-Mouse CD11c RUO
Size
0.2 mg
Cat No.
553802
APC Rat Anti-CD11b RUO
Size
0.1 mg
Cat No.
553312
564227 Rev. 2
Antibody Details
6F12
The 6F12 antibody reacts with a 7-transmembrane-domain protein, which is similar to the F4/80 macrophage antigen of the EGF-TM7 protein family and is encoded by the Emr4 gene. The FIRE protein is expressed on myeloid cells with a denditic cell (DC) developmental potential, including subsets of DC and macrophages in the spleen and lymph nodes, most resident peritoneal macrophages, many peripheral blood monocytes, and a subpopulation of bone-marrow myeloid-cell progenitors. The protein is not detected on peripheral T and B lymphocytes, and it is down-regulated on thioglycollate-elicited peritoneal macrophages and on dendritic cells activated by GM-CSF, IFN-γ, anti-CD40, and LPS. Using soluble biotinylated fusion protein, a FIRE ligand was detected on a mouse IgG+ B lymphoma cell line (A20), but not on myeloid, fibroblast, or T-cell lines, suggesting that the FIRE protein may be involved in immunoregulatory interactions between antigen-presenting cells and B lymphocytes.
564227 Rev. 2
Format Details
Alexa Fluor™ 488
Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 488
494 nm
517 nm
564227 Rev.2
Citations & References
View product citations for antibody "564227" on CiteAb
Development References (2)
-
Caminschi I, Lucas KM, O'Keeffe MA, et al. Molecular cloning of F4/80-like-receptor, a seven-span membrane protein expressed differentially by dendritic cell and monocyte-macrophage subpopulations. J Immunol. 2001; 167(7):3570-3576. (Immunogen: Immunohistochemistry, Immunoprecipitation, Western blot). View Reference
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Stacey M, Chang GW, Sanos SL, et al. EMR4, a novel epidermal growth factor (EGF)-TM7 molecule up-regulated in activated mouse macrophages, binds to a putative cellular ligand on B lymphoma cell line A20. J Biol Chem. 2002; 277(32):29283. (Biology). View Reference
564227 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.