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Alexa Fluor® 488 Mouse Anti-Human Galectin-3
Alexa Fluor® 488 Mouse Anti-Human Galectin-3
Flow cytometric analysis of Galectin-3 expression by human peripheral blood monocytes. Human peripheral blood mononuclear cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). After washing with BD Perm/Wash™ Buffer (Cat. No. 554723), the cells were stained with either Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (Cat. No. 565572; dashed line histogram) or Alexa Fluor® 488 Mouse Anti-Human Galectin-3 antibody (Cat. No. 565677; solid line histogram) at 0.25 µg/test. The fluorescence histogram showing Galectin-3 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.
Flow cytometric analysis of Galectin-3 expression by human peripheral blood monocytes. Human peripheral blood mononuclear cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). After washing with BD Perm/Wash™ Buffer (Cat. No. 554723), the cells were stained with either Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (Cat. No. 565572; dashed line histogram) or Alexa Fluor® 488 Mouse Anti-Human Galectin-3 antibody (Cat. No. 565677; solid line histogram) at 0.25 µg/test. The fluorescence histogram showing Galectin-3 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.
Product Details
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BD Pharmingen™
LGALS3; Gal-3; GAL3; GALIG; GALBP; CBP35; MAC-2; IgE-binding protein; L31
Human (QC Testing), Mouse (Reported)
Mouse BALB/c IgG1, κ
Recombinant Human Galectin-3
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2869699
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  5. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
  6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
565677 Rev. 1
Antibody Details
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B2C10

The B2C10 monoclonal antibody specifically recognizes Galectin-3 (GAL3/Gal-3) which is also known as, IgE-binding protein (εBP/IgEBP), Carbohydrate-binding protein 35 (CBP35), Galactoside-binding protein (GALBP), or  MAC-2. Galectin-3 is a 30 kDa protein that is encoded by LGALS3 (lectin, galactoside-binding, soluble, 3) and belongs to the galactoside-binding lectins family. It is expressed by a variety of cell types including monocytes/macrophages, dendritic cells, some activated lymphocytes, fibroblasts, epithelial cells, and tumor cells. Galectin-3 has diverse functions and is involved in the induction and regulation of immune responses, inflammation, cell adhesion, chemotaxis, apoptosis, angiogenesis, and fibrosis. Galectin-3 can be differentially expressed in the nucleus, cytoplasm, and surface of cells and can be secreted. Galectin-3 levels can be markedly elevated in the nuclei of proliferating cells suggesting that it may play a role in cell growth regulation. Nuclear galectin-3 has been associated with gene expression and pre-mRNA splicing. Cell surface Galectin-3 reportedly serves as a non-integrin, laminin-binding protein, suggesting its role in cell adhesion to basement membranes. The B2C10 antibody is suitable for immunohistochemical staining of paraffin-embedded tissue sections. The B2C10 antibody reportedly crossreacts with mouse Galectin-3.

        

565677 Rev. 1
Format Details
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Alexa Fluor™ 488
Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 488
Blue 488 nm
494 nm
517 nm
565677 Rev.1
Citations & References
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Development References (9)

  1. Acosta-Rodríguez EV, Montes CL, Motrán CC, et al. Galectin-3 mediates IL-4-induced survival and differentiation of B cells: functional cross-talk and implications during Trypanosoma cruzi infection.. J Immunol. 2004; 172(1):493-502. (Clone-specific: Flow cytometry). View Reference
  2. Cherayil BJ, Weiner SJ, Pillai S. The Mac-2 antigen is a galactose-specific lectin that binds IgE. J Exp Med. 1989; 170(6):1959-1972. (Biology). View Reference
  3. Cortegano I, del Pozo V, Cárdaba B, et al. Galectin-3 down-regulates IL-5 gene expression on different cell types. J Immunol. 1998; 161(1):385-389. (Biology). View Reference
  4. Haudek KC, Spronk KJ, Voss PG, Patterson RJ, Wang JL, Arnoys EJ. Dynamics of galectin-3 in the nucleus and cytoplasm.. Biochim Biophys Acta. 2010; 1800(2):181-9. (Biology). View Reference
  5. Liu FT, Hsu DK, Zuberi RI, et al. Modulation of functional properties of galectin-3 by monoclonal antibodies binding to the non-lectin domains.. Biochemistry. 1996; 35(19):6073-9. (Immunogen: Bioassay, Blocking, ELISA, Functional assay, Inhibition, Radioimmunoassay, Western blot). View Reference
  6. Mey A, Leffler H, Hmama Z, Normier G, Revillard JP. The animal lectin galectin-3 interacts with bacterial lipopolysaccharides via two independent sites. J Immunol. 1996; 156(4):1572-1577. (Biology). View Reference
  7. Newlaczyl AU, Yu LG. Galectin-3--a jack-of-all-trades in cancer.. Cancer Lett. 2011; 313(2):123-8. (Biology). View Reference
  8. Ocklenburg F, Moharregh-Khiabani D, Geffers R, et al. UBD, a downstream element of FOXP3, allows the identification of LGALS3, a new marker of human regulatory T cells.. Lab Invest. 2006; 86(7):724-37. (Clone-specific: Flow cytometry). View Reference
  9. Sano H, Hsu DK, Yu L, et al. Human galectin-3 is a novel chemoattractant for monocytes and macrophages.. J Immunol. 2000; 165(4):2156-64. (Clone-specific: Bioassay, Functional assay, Inhibition). View Reference
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565677 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.