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FITC Perforin Reagent Set

FITC Perforin Reagent Set

(RUO)
FITC Perforin Reagent Set
Flow cytometric analysis of perforin expression on human peripheral blood lymphocytes. Peripheral blood lymphocytes were permeabilized with BD Cytofix/Cytoperm™ Fixation/Permeabilization Kit (Cat. No. 554714) and stained with FITC Perforin Reagent Set (Cat. No. 556577):  FITC Anti-Human Perforin (shaded histogram) or FITC IgG2b ĸ Isotype Control (open histogram). Flow cytometric analysis was performed on a BD FACScan™ (BDIS, San Jose, CA).
Flow cytometric analysis of perforin expression on human peripheral blood lymphocytes. Peripheral blood lymphocytes were permeabilized with BD Cytofix/Cytoperm™ Fixation/Permeabilization Kit (Cat. No. 554714) and stained with FITC Perforin Reagent Set (Cat. No. 556577):  FITC Anti-Human Perforin (shaded histogram) or FITC IgG2b ĸ Isotype Control (open histogram). Flow cytometric analysis was performed on a BD FACScan™ (BDIS, San Jose, CA).
Product Details
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BD Pharmingen™
Human (QC Testing)
Intracellular staining (flow cytometry) (Routinely Tested)
RUO
AB_396470


Description

Perforin has a key role in cell-mediated cytotoxicity. It is a 70 kDa cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. CTLs are involved in eliminating virally infected cells, in anti-tumor immune responses, in allograft rejections, and in some autoimmune diseases. NK cells are important for tumor surveillance and destruction and are involved in allograft rejections. Cytotoxic cells release the contents of their cytotoxic granules, including perforin upon recognition of their target cell. In the presence of calcium, perforin forms transmembrane channels or pores in the membrane of the target cell leading to a cell death that resembles apoptosis. The ability to detect perforin-positive cells with specific antibody should be useful in identifying and understanding perforin-mediated reactions.

Clone δG9 reacts with human and bovine perforin. It does not cross-react with mouse perforin. Purified granules from the human lymphoma cell line YT were used as immunogen. Clone δG9 was initially characterized by immunoprecipitation and immunohistochemistry of frozen tissue sections. The antibody stains scattered lymphocytes in red pulp of spleen, and scattered infiltrated lymphocytes in lymphoma.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
556577 Rev. 7
Components
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Description Quantity/Size Part Number EntrezGene ID
FITC Mouse Anti-Human Perforin 100 Tests (1 ea) 51-65994X N/A
FITC Mouse IgG2b κ Isotype Control 100 Tests (1 ea) 51-66374X-2 N/A
556577 Rev. 7
Citations & References
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Development References (3)

  1. Hameed A, Fox WM, Kurman RJ, Hruban RH, Podack ER. Perforin expression in human cell-mediated luteolysis. Int J Gynecol Pathol. 1995; 14(2):151-157. (Clone-specific: Flow cytometry). View Reference
  2. Hameed A, Olsen KJ, Cheng L, Fox WM 3rd, Hruban RH, Podack ER. Immunohistochemical identification of cytotoxic lymphocytes using human perforin monoclonal antibody. Am J Pathol. 1992; 140(5):1025-1030. (Immunogen: Immunohistochemistry, Immunoprecipitation). View Reference
  3. Rukavina D, Balen-Marunic S, Rubesa G, Orlic P, Vujaklija K, Podack ER. Perforin expression in peripheral blood lymphocytes in rejecting and tolerant kidney transplant recipients. Transplantation. 1996; 61(2):285-291. (Clone-specific: Flow cytometry). View Reference
556577 Rev. 7

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.