BD Pharmingen™ APC Mouse Lineage Antibody Cocktail, with Isotype Control
Identification of CD34+ and CD34- subpopulations of hematopoietic progenitors. BALB/c bone marrow cells were treated with Mouse BD Fc Block™ Purified anti-CD16/CD32 (Cat. No. 553141/553142), stained with FITC Rat anti-Mouse CD34 (Cat. No. 553733) and either APC Mouse Lineage Isotype Control Cocktail (left panel) or APC Mouse Lineage Antibody Cocktail (right panel, Cat. No. 558074). Dead cells were excluded from analysis by staining with Propidium Iodide Staining Solution (Cat. No. 556463). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
Identification of CD34+ and CD34- subpopulations of hematopoietic progenitors. BALB/c bone marrow cells were treated with Mouse BD Fc Block™ Purified anti-CD16/CD32 (Cat. No. 553141/553142), stained with FITC Rat anti-Mouse CD34 (Cat. No. 553733) and either APC Mouse Lineage Isotype Control Cocktail (left panel) or APC Mouse Lineage Antibody Cocktail (right panel, Cat. No. 558074). Dead cells were excluded from analysis by staining with Propidium Iodide Staining Solution (Cat. No. 556463). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
Identification of CD34+ and CD34- subpopulations of hematopoietic progenitors. BALB/c bone marrow cells were treated with Mouse BD Fc Block™ Purified anti-CD16/CD32 (Cat. No. 553141/553142), stained with FITC Rat anti-Mouse CD34 (Cat. No. 553733) and either APC Mouse Lineage Isotype Control Cocktail (left panel) or APC Mouse Lineage Antibody Cocktail (right panel, Cat. No. 558074). Dead cells were excluded from analysis by staining with Propidium Iodide Staining Solution (Cat. No. 556463). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
Identification of CD34+ and CD34- subpopulations of hematopoietic progenitors. BALB/c bone marrow cells were treated with Mouse BD Fc Block™ Purified anti-CD16/CD32 (Cat. No. 553141/553142), stained with FITC Rat anti-Mouse CD34 (Cat. No. 553733) and either APC Mouse Lineage Isotype Control Cocktail (left panel) or APC Mouse Lineage Antibody Cocktail (right panel, Cat. No. 558074). Dead cells were excluded from analysis by staining with Propidium Iodide Staining Solution (Cat. No. 556463). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
Description
The APC Mouse Lineage Antibody Cocktail has been designed to react with cells from the major hematopoietic lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, NK cells, erythrocytes, and granulocytes. This pre-diluted Cocktail of five APC-conjugated antibodies is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include clone 145-2C11, which recognizes Mouse CD3e; M1/70, which recognizes CD11b; RA3-6B2, which recognizes CD45R/B220; TER-119, which recognizes Ly-76, mouse erythroid cells; and RB6-8C5, which recognizes Ly-6G and Ly-6C. APC Mouse Lineage Isotype Control Cocktail contains equivalent concentrations of isotype-matched negative-control immunoglobulin. Additional fluorochrome-labeled reagents may be combined with the APC Mouse Lineage Antibody Cocktail, and the APC Mouse Lineage Isotype Control Cocktail, to further characterize hematopoietic progenitor subpopulations.
Preparation And Storage
Recommended Assay Procedures
20 µl of the antibody or isotype control cocktail is sufficient to stain each sample of 10^6 leukocytes for flow cytometric analysis. We recommend the use of Mouse BD Fc Block™ purified anti-mouse CD16/Cd32 (Cat. No. 553141/553142) for optimal staining. The APC Mouse Lineage Antibody Cocktail can be used to deplete cells bearing the hematopoietic lineage markers by flow cytometric or immunomagnetic (using BD™ IMag anti-Allophycocyanin (APC) Particles - DM, Cat. No. 557932) sorting. The APC fluorochrome is excited by laser lines from 595 to 647 nm, and its emission is collected in a detector for fluorescence wavelengths between 640 and 680 nm.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
| Description | Quantity/Size | Part Number | EntrezGene ID |
|---|---|---|---|
| N/A | 100.0 | N/A | N/A |
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.