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      Antibody Cocktail (IFN-γ FITC, CD69 PE, CD4 PerCP-Cy™5.5)

      BD FastImmune™ Antibody Cocktail (IFN-γ FITC, CD69 PE, CD4 PerCP-Cy™5.5)

      (RUO (GMP))
      Product Details
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      BD FastImmune™
      Human
      Flow cytometry
      RUO (GMP)
      Phosphate buffered saline with BSA, beta-lactoglobulin, and 0.1% sodium azide.


      Description

      Anti-Hu–IFN-γ, clone 25723.11, is derived from the hybridization of P3X-63-Ag8.653 mouse myeloma cells with lymph node cells from BALB/c mice immunized with recombinant human IFN-γ.

      CD69, clone L78, is derived from hybridization of Sp2/0-Ag14 mouse myeloma cells with lymph node cells from BALB/c mice immunized with a CD8+ alloantigen-directed cytotoxic T-lymphocyte (CTL) cell line.

      CD4, clone SK3, is derived from hybridization of NS-1mouse myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood T lymphocytes.

      Anti-Human Interferon-γ (Anti-Hu–IFN-γ) recognizes a 20- to 25-kilodalton (kDa) glycoprotein.

      CD69 recognizes a very early human activation antigen. The CD69 antigen is a surface homodimer formed by the association of 28-kDa and 32-kDa chains that are held together by disulfide bridges.

      The CD4 antigen, with a molecular weight of 55 kDa, is present on T-helper/inducer lymphocytes and monocytes.


      Anti-Hu–IFN-γ, clone 25723.11, is derived from the hybridization of P3X-63-Ag8.653 mouse myeloma cells with lymph node cells from BALB/c mice immunized with recombinant human IFN-γ.

      CD69, clone L78, is derived from hybridization of Sp2/0-Ag14 mouse myeloma cells with lymph node cells from BALB/c mice immunized with a CD8+ alloantigen-directed cytotoxic T-lymphocyte (CTL) cell line.

      CD4, clone SK3, is derived from hybridization of NS-1mouse myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood T lymphocytes.

      Anti-Human Interferon-γ (Anti-Hu–IFN-γ) recognizes a 20- to 25-kilodalton (kDa) glycoprotein.

      CD69 recognizes a very early human activation antigen. The CD69 antigen is a surface homodimer formed by the association of 28-kDa and 32-kDa chains that are held together by disulfide bridges.

      The CD4 antigen, with a molecular weight of 55 kDa, is present on T-helper/inducer lymphocytes and monocytes.

      Preparation And Storage

      Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

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      340962 Rev. 1
      Components
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      Description Clone Isotype EntrezGene ID
      IFN-γ FITC TgMab-2 IgG1, κ N/A
      CD69 PE 2D7/Basophils IgG1, κ N/A
      CD4 PerCP-CY5.5 SK3 IgG1, κ N/A
      340962 Rev. 1
      Citations & References
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      Development References (38)

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      33. Testi R, Philips JH, Lanier LL. Leu-23 induction as an early marker for functional CD3/T cell antigen receptor triggering: requirement for receptor cross-linking, prolonged elevation of intracellular (Ca ++ ), and stimulation of protein kinase C. J Immunol. 1988; 2:1214. (Biology).
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      View All (38) View Less
      340962 Rev. 1

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      For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

       

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