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      Purified Mouse anti-BLNK (pY84)
      Purified Mouse anti-BLNK (pY84)
      Western blot analysis of BLNK (pY84) in human Burkitt's lymphoma.  Lysates from control (lanes 1-3) and hydrogen peroxide-activated (lanes 4-6) Ramos  cells were probed with purified mouse anti-BLNK (pY84) monoclonal antibody) at concentrations of 0.125 (lanes 1 and 4), 0.0625 (lanes 2 and 5), and 0.032 µg/ml (lanes 3 and 6).  BLNK (pY84) is identified as a band of about 68 kDa in the treated cells.
      Purified Mouse anti-BLNK (pY84)
      Western blot analysis of BLNK (pY84) in human Burkitt's lymphoma.  Lysates from control (lanes 1-3) and hydrogen peroxide-activated (lanes 4-6) Ramos  cells were probed with purified mouse anti-BLNK (pY84) monoclonal antibody) at concentrations of 0.125 (lanes 1 and 4), 0.0625 (lanes 2 and 5), and 0.032 µg/ml (lanes 3 and 6).  BLNK (pY84) is identified as a band of about 68 kDa in the treated cells.
      Product Details
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      BD Pharmingen™
      Human (QC Testing), Mouse (Reported)
      Mouse BALB/c IgG2b, κ
      Phosphorylated Human BLNK Peptide
      Western blot (Routinely Tested)
      68 kDa
      0.5 mg/ml
      AB_647263
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      558366 Rev. 4
      Antibody Details
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      J117-1278

      B cell activation is initiated by crosslinking the B cell receptor, which leads to activation of non-receptor protein tyrosine kinases (PTK), including Btk, Syk, and three Src kinases, Fyn, Lyn, and Blk.  Activated PTKs then phosphorylate multiple cellular proteins involved in B lymphocyte signaling.  Syk is responsible for the tyrosine phosphorylation of B cell linker protein (BLNK), a member of the SLP-76 family of adapter proteins.  Phosphorylation of human BLNK at tyrosines 84, 178, and 189 (Y84, Y178, and Y189) creates docking sites for PLCγ2, leading to the activation of downstream signaling pathways.  

      The J117-1278 monoclonal antibody recognizes the phosphorylated Y84 of BLNK.

      558366 Rev. 4
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      558366 Rev.4
      Citations & References
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      Development References (5)

      1. Chiu CW, Dalton M, Ishiai M, Kurosaki T, Chan AC. BLNK: molecular scaffolding through 'cis'-mediated organization of signaling proteins. EMBO J. 2002; 21:6461-6472. (Biology).
      2. Espeli M, Mancini S, Breton C, Poirier F, Schiff C . Impaired B-cell development at the pre-Bll-cell stage in galectin-1-deficient mice due to inefficient pre-Bll/stromal cell interactions. Blood. 2009; 113(23):5878-5886. (Clone-specific: Flow cytometry). View Reference
      3. Janssen E, Zhang W. Adaptor proteins in lymphocyte activation. Curr Opin Immunol. 2003; 15:269-276. (Biology). View Reference
      4. Pighi C, Gu T, Dalai I, et al. Phospho-proteomic analysis of mantle cell lymphoma cells suggests a pro-survival role of B-cell receptor signaling. Cell Oncol. 2011; 34(2):141-153. (Clone-specific: Flow cytometry, Western blot). View Reference
      5. Wu JN, Koretzky GA. The SLP-76 family of adapter proteins. Semin Immunol. 2004; 16:379-393. (Biology). View Reference
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      558366 Rev. 4

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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