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Purified Mouse Anti-TEF-1
Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse (Tested in Development)
Mouse IgG1
Human TEF-1 aa. 86-199
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
53 kDa
250 µg/ml
AB_398238
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610923 Rev. 1
Antibody Details
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31/TEF-1

TEF-1 (transcription enhancer factor-1) is a member of a family of transcription factors that contain an evolutionarily conserved DNA binding domain (DBD), TEA/ATTS. TEF-1 was identified as a HeLa cell transcriptional activator that bound, via its TEA domain, to the GT-IIC and Sph enhancer elements. Comparison of various enhancers indicates that TEF-1 binds to highly degenerate DNA sequences and cooperatively interacts with tandem repeats of its binding sites, which increases its enhancer activity. However, the DNA binding activities of TEF-1 are modulated by sequences outside the DBD. The N-terminal basic portion of TEF-1 contains the DBD. The C-terminal portion contains the transactivation domain and a zinc finger-like motif that is thought to be involved in protein dimerization or interaction with additional transcription factors. TEF-1 induced transcription requires a limiting transcriptional intermediary factor (TIF) and a TATA-binding protein associated factor (hTAFIIs). Although TEF-1 is expressed ubiquitously during embryogenesis, its expression is limited to adult kidney, heart, brain, skeletal muscle, and lungs. Disruption of murine TEF-1 gene results in fetal death.

610923 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610923 Rev.1
Citations & References
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Development References (5)

  1. Deshpande N, Chopra A, Rangarajan A, Shashidhara LS, Rodrigues V, Krishna S. The human transcription enhancer factor-1, TEF-1, can substitute for Drosophila scalloped during wingblade development. J Biol Chem. 1997; 272(16):10664-10668. (Biology). View Reference
  2. Gupta MP, Amin CS, Gupta M, Hay N, Zak R. Transcription enhancer factor 1 interacts with a basic helix-loop-helix zipper protein, Max, for positive regulation of cardiac alpha-myosin heavy-chain gene expression. Mol Cell Biol. 1997; 17(7):3924-3936. (Biology). View Reference
  3. Jacquemin P, Hwang JJ, Martial JA, Dolle P, Davidson I. A novel family of developmentally regulated mammalian transcription factors containing the TEA/ATTS DNA binding domain. J Biol Chem. 1996; 271(36):21775-21785. (Biology). View Reference
  4. Ueyama T, Zhu C, Valenzuela YM, Suzow JG, Stewart AF. Identification of the functional domain in the transcription factor RTEF-1 that mediates alpha 1-adrenergic signaling in hypertrophied cardiac myocytes. J Biol Chem. 2000; 275(23):17476-17480. (Clone-specific: Gel shift). View Reference
  5. Xiao JH, Davidson I, Matthes H, Garnier JM, Chambon P. Cloning, expression, and transcriptional properties of the human enhancer factor TEF-1. Cell. 1991; 65(4):551-568. (Biology). View Reference
View All (5) View Less
610923 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

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