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Purified Mouse Anti-Rab5
Product Details
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BD Transduction Laboratories™
Human (QC Testing), Dog (Tested in Development)
Mouse IgG2a
Human Rab5 aa. 1-215
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development)
25 kDa
250 µg/ml
AB_398048
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610725 Rev. 2
Antibody Details
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1/Rab5

Rab5 is a low molecular weight GTP-binding protein that plays a role in endocytic vesicle traffic. Like other Rab proteins, Rab5 has C-terminal cysteine residues that are post-translationally modified by geranylgeranylation which is critical for its membrane targeting. Rab5 is associated with early endosome and plasma membranes and evidence suggests that Rab5 regulates early endosome fusion. The GTP/GDP cycle controls shuttling of Rab proteins between the cytosol and membranes. In vitro, Rab5 proteins are removed from membranes by a GDP dissociation inhibitor protein (rabGDI) which leads to the formation of a cytosolic Rab5-rabGDI complex. Rab5 may insert into membranes by a multistep process in a which a transient GDP-Rab5 intermediate is formed and converted into GTP-Rab5 that subsequently enters the acceptor membrane and releases rabGDI into the cytosol.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610725 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610725 Rev.2
Citations & References
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Development References (4)

  1. Li G, Stahl PD. Structure-function relationship of the small GTPase rab5. J Biol Chem. 1993; 268(32):24475-24480. (Biology). View Reference
  2. Sanford JC, Pan Y, Wessling-Resnick M. Prenylation of Rab5 is dependent on guanine nucleotide binding. J Biol Chem. 1993; 268(32):23773-23776. (Biology). View Reference
  3. Stenmark H, Vitale G, Ullrich O, Zerial M. Rabaptin-5 is a direct effector of the small GTPase Rab5 in endocytic membrane fusion. Cell. 1995; 83(3):423-432. (Biology). View Reference
  4. Ullrich O, Horiuchi H, Bucci C, Zerial M. Membrane association of Rab5 mediated by GDP-dissociation inhibitor and accompanied by GDP/GTP exchange. Nature. 1994; 368(6467):157-160. (Biology). View Reference
View All (4) View Less
610725 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

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